Plasma/Serum Circulating DNA Isolation Mini Kit (Slurry Format): Rapid, High-Yield Cell-Free DNA Extraction
Norgen’s Plasma/Serum Circulating DNA Isolation Mini Kit (Slurry Format) provides a rapid, robust, and highly efficient method for the purification of fragmented free-circulating DNA (cf-DNA) from human plasma or serum samples ranging from 50 µL to 400 µL. Free-circulating nucleic acids in clinical samples serve as vital biomarkers for cancer monitoring, non-invasive prenatal testing (NIPT), and various infectious disease states. However, isolating these low-abundance, highly fragmented targets remains a significant laboratory challenge. This kit effectively resolves this bottleneck by capturing the full spectrum of circulating DNA while completely removing contaminating cellular debris, proteins, and enzymatic inhibitors that often compromise downstream results.
The purification technology relies on spin column chromatography utilising Norgen’s proprietary silicon carbide resin matrix as an exceptional ion exchanger. Unlike conventional spin column protocols that require bulky, specialised extension tubes to accommodate larger liquid volumes, this innovative slurry format enables convenient, direct binding in a single tube step. Cellular contaminants and PCR inhibitors are thoroughly removed via targeted washing stages, allowing highly concentrated, active circulating DNA to be eluted in less than 30 minutes for direct deployment in sensitive molecular applications.
Key Benefits:
- Versatile Sample Inputs: Flexible volume handling. Enables successful isolation of circulating DNA from a broad range of human plasma or serum sample volumes, from 50 µL up to 400 µL.
- Slurry-Based Workflow: No extension tubes required. The advanced slurry design eliminates the need for specialised extension attachments, streamlining sample handling and minimising the risk of sample-to-sample carryover.
- Rapid Processing Speed: High-throughput efficiency. The entire purification protocol is completed in less than 30 minutes, significantly reducing hands-on time and accelerating laboratory throughput.
- Comprehensive DNA Recovery: Full spectrum isolation. efficiently captures all sizes of circulating DNA fragments down to 50 bp, while also facilitating the co-isolation of viral and bacterial DNA from plasma, serum, and milk samples.
- Downstream Compatibility: Inhibitor-free elution. Yields highly pure DNA that is fully compatible with sensitive downstream molecular applications including PCR, qPCR, methylation-sensitive PCR, and Next-Generation Sequencing (NGS).
Technical Insights
Comparative evaluation demonstrates that this slurry-based purification matrix delivers exceptional sensitivity and yield compared to traditional column methods. In qPCR amplification assays targeting the human 5S gene, DNA isolated using this kit consistently achieves lower cycle threshold (Ct) values across multiple sample volumes. This confirms superior recovery of low-abundance, highly fragmented cell-free DNA targets without cross-contamination or inhibitor carryover, preserving sample integrity for critical diagnostic research.
Specifications
- Catalogue Number: P4-0124
- Sample Type: Human plasma, serum, and milk samples (fresh or frozen)
- Sample Input Range: 50 µL to 400 µL
- Minimum Input Volume: 50 µL
- Maximum Input Volume: 400 µL
- Elution Volume: 100 µL
- Size of Purified DNA: ≥ 50 bp (recovers small fragments under 1000 bp)
- Time to Complete Purification: Less than 30 minutes
- Kit Size: 50 preparations
- Storage Conditions: Store all buffers tightly sealed at room temperature (15–25°C)
- Product Stability: Stable for up to 2 years from the date of shipment under proper storage conditions