Plant/Fungi Total RNA Purification Kit: Rapid & Phenol-Free RNA Isolation
The Plant/Fungi Total RNA Purification Kit provides an innovative and rapid method for the isolation and purification of total RNA, including virus and viroid RNA, from a wide range of plant and filamentous fungal species. Designed to overcome the challenges associated with difficult, polyphenol-rich samples such as pine needles, grape leaves, and strawberry tissues, this kit effectively extracts all sizes of RNA. From large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), researchers can rely on exceptional yield and integrity for the most demanding molecular biological applications.
The purification mechanism is based on advanced spin-column chromatography utilising a proprietary resin as the separation matrix, which preferentially binds nucleic acids dependent upon ionic concentrations. By employing a robust lysis buffer containing chaotropic denaturants, RNases and proteases are rapidly inactivated, completely bypassing the need for hazardous phenol or chloroform extractions and removing the requirement for liquid nitrogen during homogenisation. This highly optimised technology effectively eliminates cellular debris and contaminants, delivering pristine RNA that is perfectly suited for real-time PCR, Northern blotting, Next Generation Sequencing (NGS), and expression array analysis.
Key Benefits:
- Phenol-Free Extraction: Eliminates hazardous chemicals. Total RNA is isolated safely and efficiently without the use of harmful organic solvents such as phenol or chloroform, fostering a safer laboratory environment.
- Comprehensive RNA Recovery: Isolate all RNA sizes. Purify the complete RNA spectrum, from large mRNA and rRNA down to small RNA species such as miRNA and siRNA (< 200 nt), ensuring no critical genetic data is left behind.
- Handles Challenging Samples: Robust lysis capability. The specially formulated lysis buffer successfully processes difficult, polysaccharide-rich plant materials where conventional methods and competitor kits frequently fail.
- Exceptional Purity: Optimised for sensitive assays. Delivers uncompromised RNA purity with 260/230 ratios consistently above 2.0, providing flawless templates for qRT-PCR, reverse transcription PCR, and transcriptomics.
- Rapid Workflow: Process samples in thirty minutes. The streamlined spin-column format is remarkably fast and convenient, allowing researchers to complete 10 purifications in just half an hour without relying on liquid nitrogen.
Technical Insights
High Purity and Integrity from Difficult Botanical Samples
Comparative analyses demonstrate that this purification kit consistently outperforms market alternatives when isolating RNA from recalcitrant plant tissues. Spectrophotometric data indicates superior purity, yielding 260/230 ratios typically above 2.0, whereas conventional methods often struggle with ratios below 1.2 due to carbohydrate and phenolic compound carryover.
Furthermore, RT-qPCR validation confirms the absolute integrity of the extracted RNA, successfully amplifying targets such as EF1-α from challenging inputs like apple, peach, and pine needle samples without inhibition.
Specifications
- Maximum Column Binding Capacity: 50 µg
- Maximum Column Loading Volume: 650 µL
- Size of RNA Purified: All sizes, including small RNA (< 200 nt)
- Maximum Amount of Starting Material: 50 mg (Plant Tissues), 1 x 10^6 cells (Plant Cells), 50 mg wet weight (Fungi)
- Time to Complete 10 Purifications: 30 minutes
- Average Yield (50 mg input): Tomato/Tobacco Leaves (60 µg), Grape Leaves (35 µg), Plum Leaves (32 µg)
- Format: Mini Spin Column (Also available in 96-well high-throughput format)
- Storage Conditions: All components should be stored securely at room temperature.
- Shelf Life: Stable for up to 2 years from the date of shipment.