NGS Normalisation 96-Well Kit: High-Throughput PCR Clean-up and Concentration Control
The NGS Normalisation 96-Well Kit is a high-performance solution designed for the simultaneous clean-up and concentration normalisation of PCR amplicons in a high-throughput format. In Next-Generation Sequencing (NGS) workflows, achieving a uniform concentration of DNA across multiple samples is critical for balanced library pooling and optimal flow cell loading. This kit eliminates the need for time-consuming fluorometric quantification and manual dilution by using a sophisticated binding-capacity-limited matrix to ensure that every well elutes a consistent, normalised amount of purified DNA.
The technology is based on 96-well column chromatography utilising a proprietary resin. Each well is engineered with a specific, limited binding capacity; when an excess of PCR product is loaded, the resin reaches saturation, effectively “capping” the amount of DNA that can be bound. During the rapid 20-minute protocol, PCR by-products—including primers, dimers, enzymes, and unincorporated nucleotides—are removed through a series of wash steps. The result is a highly pure, normalised PCR amplicon ready for direct use in library preparation, sequencing, and other sensitive genomic applications.
Key Benefits:
- Dual-Action Processing: Simultaneous clean-up and normalisation. Combines the removal of inhibitory PCR by-products with concentration control in a single, streamlined workflow.
- High-Throughput Efficiency: Process 96 samples in under 20 minutes. The centrifugation-based protocol is designed for speed and ease of use, significantly reducing hands-on time compared to traditional methods.
- Consistent Yields: Optimised binding capacity. The proprietary resin ensures that each well elutes an equal concentration of PCR product, ensuring uniformity across the entire plate.
- Superior Purity: Complete by-product removal. Effectively eliminates primer dimers and small fragments that can interfere with sequencing quality and data accuracy.
- Application Ready: Generous elution volume. Elutes in 100 µL, providing sufficient material for multiple downstream assays or long-term archiving.
Technical Insights
Normalisation through Saturation Chromatography
The fundamental principle of this kit is “normalisation by saturation.” By providing a separation matrix with a precise, finite number of binding sites, the system ensures that regardless of the initial concentration of the input PCR product (provided it is in excess), the final eluted concentration remains constant. This non-magnetic, column-based approach provides high reproducibility and is easily integrated into standard laboratory workflows without the need for specialised magnetic stands or liquid handling robotics.
Specifications
- Format: 96-Well Filter Plate
- Processing Time: Less than 20 minutes
- Binding Technology: Proprietary resin-based (Limited capacity)
- Elution Volume: 100 µL
- Input Type: PCR amplicons (Standard and high-yield)
- Applications: NGS Library Prep, Sanger Sequencing, and High-Throughput Screening