Urine Cell-Free Circulating RNA Purification Maxi Kit: High-Yield Transcriptomic Recovery from Large-Volume Urine
The Urine Cell-Free Circulating RNA Purification Maxi Kit provides a fast, reliable, and convenient method to purify and concentrate high-quality, inhibitor-free cell-free circulating RNA (cfc-RNA) from large urine volumes. As RNA species in urine are often present at extremely low concentrations, this kit is specifically engineered to handle inputs ranging from 10 mL to 30 mL. It enables the robust recovery of cfc-RNA, exosomal RNA, and viral RNA from fresh, preserved, or frozen samples, providing a critical tool for non-invasive liquid biopsy and biomarker discovery.
The purification mechanism utilizes a sophisticated two-column system designed for maximum concentration efficiency. The process begins with a large-volume column that handles the initial urine input, effectively capturing the total RNA fraction. This is followed by a secondary concentration step on a mini column, allowing for a final elution volume as low as 50 µL to 100 µL. By avoiding hazardous phenol/chloroform extractions and tedious protease treatments, the kit ensures the isolated RNA maintains the highest integrity for sensitive downstream molecular analyses.
Key Benefits:
- Superior Concentration Efficiency: Maximises yield from dilute samples. Processes up to 30 mL of urine and elutes into a highly concentrated 50 µL volume, ensuring detection of low-abundance transcripts.
- Comprehensive RNA Capture: Recovers diverse RNA species. Efficiently isolates all sizes of circulating RNA, including exosomal RNA and viral RNA, providing a complete transcriptomic profile.
- Streamlined Two-Column Protocol: Optimised for large volumes. The transition from a large-volume capture column to a mini concentration column simplifies the handling of bulky samples without sacrificing speed.
- Assay-Ready Purity: Eliminates urinary inhibitors. Delivers high-purity RNA that is fully compatible with NGS, RT-qPCR, Northern blotting, and methylation-sensitive assays.
Technical Insights
Two-Stage Concentration Workflow
The diagram illustrates the transition from large-volume urine processing to high-concentration elution. By utilizing a dual-column approach, the system effectively bridges the gap between bulky clinical samples and the small-volume requirements of modern genomic platforms.
Specifications
- Catalogue No: P4-0359
- Input Volume: 10 mL – 30 mL
- Sample Type: Fresh, preserved, or frozen urine
- Elution Volume: 50 µL – 100 µL
- Downstream Compatibility: NGS, PCR, qPCR, RT-PCR, Northern Blotting, and Expression Arrays
- Processing Time: Rapid workflow (Phenol/Chloroform free)