Urine Cell-Free Circulating RNA Purification Midi Kit: Precision RNA Recovery from Mid-Volume Urine Samples
The Urine Cell-Free Circulating RNA Purification Midi Kit offers a fast, reliable, and convenient solution for the isolation and concentration of high-quality, inhibitor-free cell-free circulating RNA (cfc-RNA). Specifically optimised for urine volumes ranging from 2 mL to 10 mL, this kit is an essential tool for researchers investigating non-invasive biomarkers, including exosomal RNA and viral RNA. It provides a robust workflow for recovering low-abundance RNA species from fresh, preserved, or frozen urine specimens, bridging the gap between standard mini kits and large-scale maxi preparations.
The kit employs a unique two-stage purification process to ensure maximum recovery and concentration. The initial phase utilizes a large-capacity column to capture the RNA from the primary urine input, which is then transferred to a secondary mini column for final concentration. This dual-column technology allows for elution into small volumes of 50 µL to 100 µL, effectively concentrating the RNA for high-sensitivity detection. By removing the need for hazardous phenol/chloroform or time-consuming protease treatments, the kit preserves the integrity of all RNA sizes, including small microRNAs and larger viral transcripts.
Key Benefits:
- Dual-Column Concentration: Maximises sample density. Transitioning from a midi capture column to a mini elution column ensures that RNA from up to 10 mL of urine is highly concentrated for downstream use.
- Broad RNA Fraction Capture: Isolates all RNA species. Efficiently recovers cell-free circulating RNA, exosomal RNA, and viral RNA, providing a comprehensive snapshot of the urinary transcriptome.
- Safe, Rapid Workflow: No toxic organic solvents. The phenol-free and chloroform-free protocol simplifies laboratory safety requirements and significantly reduces the total time from sample to purified RNA.
- Inhibitor-Free Eluate: Optimised for sensitive assays. Effectively eliminates urinary inhibitors, ensuring the purified RNA is fully compatible with NGS, RT-qPCR, and methylation-sensitive applications.
Technical Insights
Efficient Two-Stage Purification
The diagram highlights the sequential column process that enables the processing of larger urine volumes without compromising the final concentration. This workflow ensures that even the most dilute RNA biomarkers are captured and concentrated into a volume suitable for modern molecular platforms.
Specifications
- Catalogue No: P4-0358
- Input Volume: 2 mL – 10 mL
- Sample Compatibility: Fresh, preserved, or frozen urine
- Elution Volume: 50 µL – 100 µL
- Technology: Two-stage spin column chromatography
- Downstream Compatibility: NGS, PCR, qPCR, Northern Blotting, and RNase protection assays