Urine Exosome Purification & RNA Isolation Midi Kit – 58700

Exosome Purification and RNA Isolation Midi Kit constitutes an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different urine sample volumes ranging from 2ml to 10ml. The purification is based on spin column chromatography that employs proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µl to 100 µl. The purified RNA is free from any protein-bound circulating RNA and of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.