Exosomal RNA Isolation Kit: Rapid, Phenol-Free Extraction of Total EV RNA
The Exosomal RNA Isolation Kit (P4-0197) addresses the critical need for high-purity RNA recovery from extracellular vesicles (EVs). Traditional methods often suffer from low yields or chemical carryover, which can significantly hinder downstream molecular analysis. This kit provides a streamlined, professional solution for researchers seeking to isolate and concentrate exosomal RNA from samples previously processed using Norgen’s specific urine or plasma/serum exosome purification technologies, ensuring high-integrity results for sensitive applications.
This system utilises a proprietary resin-based chromatography to selectively bind and concentrate RNA from intact or purified exosomes. Unlike conventional organic extraction methods, it completely eliminates the requirement for phenol, chloroform, or protease treatments, significantly reducing processing time and environmental hazards. The technology meticulously captures a comprehensive range of RNA species, including microRNA (miRNA) and other small RNAs, ensuring a representative profile of the exosomal cargo for transcriptomic profiling.
Key Benefits:
- Chemical Purity: Phenol and Chloroform-Free. The protocol eliminates hazardous organic solvents, preventing chemical carryover that can inhibit downstream enzymatic reactions.
- Comprehensive Capture: Total RNA Recovery. Efficiently isolates all RNA sizes, from large mRNA down to critical microRNA species, providing a complete picture of the exosome profile.
- High Sensitivity: Optimised Concentration. Allows for flexible elution volumes as low as 50 µl to 100 µl, resulting in highly concentrated samples ready for low-abundance target detection.
- Streamlined Workflow: Rapid and Simple. No specialised equipment, protein precipitation reagents, or extension tubes are required, allowing for fast and reliable sample preparation.
Technical Insights
Figure 1. Isolation of RNA from exosomes purified from different urine volumes.
Norgen’s Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different urine volumes purified using Norgen’s Urine Exosome Purification Kits (Cat# 57700, 57800 and 57900). 2 μL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated urinary exosomal miR-30a. (A) The average Ct value for urinary exosomal miR-30a is linearly decreasing with increasing the sample input volume. B) The relative amount of the urinary exosomal miR-30a shows excellent linearity with a percentage of recovery of more than 90%.
Figure 2. Isolation of RNA from exosomes purified from different plasma volumes.
Norgen’s Exosomal RNA Isolation Kit (Cat# 58000) was used to isolate RNA from exosomes isolated from different plasma volumes purified using Norgen’s Plasma/Serum Exosome Purification Kits (Cat# 57400, 57500 and 57600). 2 μL of the isolated RNA was then used as the template in RT-qPCR reactions to assess the amplification of the isolated plasma exosomal miR-26a. (A) The average Ct value for plasma exosomal miR-26a is linearly decreasing with increasing the sample input volume. B) The relative amount of the plasma exosomal miR-26a shows excellent linearity with a percentage of recovery of more than 90%.
Specifications
- Catalogue Number: P4-0197 (Norgen 58000)
- Input Compatibility: Exosomes purified from Urine or Plasma/Serum
- Elution Volume: 50 µl – 100 µl
- RNA Species Recovered: All sizes, including miRNA and small RNA
- Extraction Chemistry: Proprietary Resin (Phenol-free)
- Downstream Applications: RT-qPCR, Northern Blotting, NGS, and Microarrays