FastGene® Plasmid Mini Prep Kit: Complete & Rapid DNA Isolation
The FastGene® Plasmid Mini Prep Kit is engineered for the rapid, small-scale isolation and purification of plasmid DNA from bacterial cultures. Optimised to handle both high-copy and low-copy vectors efficiently, this kit addresses the need for speed without compromising on purity. The resulting plasmid DNA is eluted in a low-salt Tris buffer, ensuring it is of high quality and immediately ready for sensitive downstream applications such as cloning, Sanger sequencing, PCR, transformation, and restriction analysis.
Distinct from standard isolation kits, the FastGene® system provides a comprehensive “all-in-one” solution to streamline laboratory workflows. The kit includes ready-to-use LB-Broth capsules, significantly reducing preparation time for bacterial culture media. By combining a fast elution protocol with high-binding column technology, researchers can achieve consistent, high-yield results faster than with comparable competitor kits, maximising laboratory efficiency.
Key Benefits:
- Complete Workflow Solution: Includes LB-Broth capsules. Each kit is supplied with pre-measured LB-Broth capsules; simply dissolve one capsule in 40 mL of water and autoclave to prepare the necessary growth medium immediately.
- Rapid Protocol: Faster preparation time. The optimised buffer system and column design allow for a quicker isolation process compared to standard competitor protocols, saving valuable bench time.
- High Quality Output: Sequencing-grade purity. Delivers high-yield plasmid DNA free from genomic DNA and cellular contaminants, suitable for capillary sequencing and transformation.
- Versatile Performance: High and low copy compatibility. Effectively isolates DNA from various plasmid copy numbers, ensuring flexibility across different experimental designs.
Technical Insights
Integrated Media Preparation
The inclusion of LB-Broth capsules simplifies the initial steps of plasmid preparation, ensuring fresh and consistent culture media for every experiment.
Fig. 1: Yield Comparison
pBluescript plasmid DNA was isolated from a 1.4 ml E. coli culture (DH5α in LB medium) according to the recommended procedures of different kits and eluted in 50 µl elution buffer. 2 µl of each eluate were loaded on a 0.7% TAE agarose gel. The FastGene® kit demonstrates equal or superior yield compared to suppliers with more time-consuming protocols.
Fig. 2: Sequencing Validation
Typical fluorescent capillary sequencing result from pBluescript plasmid DNA purified using the FastGene® Plasmid Mini Kit. Cycle sequencing reaction was performed on an ABI3100 sequencer. 1 µl of purified plasmid DNA was used as a template. Dye terminators were removed by FastGene® Dye Terminator Removal Kit.
Specifications
- Pack Size: 100 Preps (P4-0502) or 300 Preps (P4-0504)
- Sample Input: ~1.4 mL bacterial culture
- Elution Volume: 50 µl
- Downstream Applications: Cloning, Sequencing, PCR, Transformation, Restriction Analysis
- Buffer Type: Low-salt Tris buffer
- Included Components: LB-Broth Capsules, Buffers, Spin Columns