FastGene® 2x IC Green Universal qPCR Mix (Fluorescein)

FastGene® 2x IC Green Universal qPCR Mix (Fluorescein): Precision and Versatility

FastGene® 2x IC Green Universal qPCR Mix (Fluorescein) is a high-performance, ready-to-use master mix designed for intercalating dye-based real-time PCR. It addresses common laboratory challenges such as inconsistent amplification and low sensitivity, providing a robust solution for quantifying DNA across a wide range of templates. By integrating all necessary components into a single mix, it reduces pipetting errors and ensures high reproducibility in gene expression and diagnostic workflows.

The technology utilises a sophisticated hot-start DNA polymerase coupled with an optimised buffer system to maximise specificity and yield. The inclusion of a green intercalating dye allows for real-time monitoring of product accumulation, while the Fluorescein passive reference dye ensures compatibility with specific thermal cyclers requiring this signal for well-to-well normalisation. This combination ensures that even low-copy number targets are detected with high precision and clarity.

Key Benefits:

• Universal Compatibility: Seamless integration with various platforms. Optimised for use with all real-time PCR instruments requiring Fluorescein as a passive reference.
• High Sensitivity: Detect low-copy targets reliably. Engineered to provide a wide dynamic range, ensuring accurate quantification of even the most challenging samples.
• Enhanced Specificity: Minimise non-specific amplification. The hot-start enzyme technology prevents primer-dimer formation and mispriming during reaction setup.
• Rapid Protocols: Optimised for fast cycling. The buffer chemistry is designed to support high-speed thermal cycling protocols without compromising data quality.

Specifications

• Format: 2x Master Mix
• Detection Method: Intercalating Green Dye
• Reference Dye: Fluorescein
• Hot-Start Activation: Chemical/Antibody-mediated
• Storage: -20°C (Protect from light)
• Shelf Life: 12 months from date of manufacture