UreaGel 29:1 Concentrate: High-Resolution Denaturing PAGE Solution
UreaGel 29:1 Concentrate is a premium, pre-mixed acrylamide solution designed for the preparation of denaturing polyacrylamide gels used in high-resolution nucleic acid analysis. Preparing manual sequencing gels often involves handling hazardous acrylamide powders and managing complex urea concentrations, which can lead to inconsistency and safety risks. UreaGel 29:1 (EC-828) simplifies this process by providing a stabilized, ready-to-use concentrate that ensures reproducible gel matrices for DNA sequencing, RNA analysis, and mutation detection (such as SSCP).
The UreaGel 29:1 system works by integrating acrylamide and bis-acrylamide at a precise 29:1 ratio within a 7.5M urea deionised aqueous solution. When combined with UreaGel Diluent and UreaGel Buffer, it allows researchers to easily adjust the monomer concentration for any commonly used gel formulation up to 20%. This liquid-based system eliminates the health hazards associated with airborne acrylamide dust and ensures that the final gel is consistently crystal clear and certified nuclease-free, preserving the integrity of sensitive genetic samples.
Key Benefits:
- Unmatched Consistency: Pre-mixed for reproducibility. Eliminates the variability of manual weighing, providing identical 29:1 cross-linking ratios every time.
- Enhanced Lab Safety: Zero-dust liquid format. Reduces the risk of neurotoxic acrylamide inhalation by replacing powdered reagents with a stabilized aqueous solution.
- Optimised Resolution: 7.5M Urea concentration. Maintained throughout the system to ensure complete denaturation of DNA and RNA for sharp, high-resolution banding.
- Nuclease-Free Assurance: Certified for sensitivity. Strictly tested to ensure the absence of RNase and DNase contamination, protecting your most critical samples.
- Versatile Gel Percentages: Customisable formulations. Allows for easy preparation of any gel concentration from 4% to 20% by simply adjusting the ratio of diluent and buffer.
Technical Insights
System Components and Preparation
The UreaGel 29:1 System is a modular three-component kit. By varying the volumes of UreaGel Concentrate, Diluent, and Buffer, researchers can precisely control the gel percentage. For example, an 8% gel requires a specific mixture (Vc = (Vt)(X)/25) to maintain the optimal 7.5M urea concentration and 1X TBE buffer strength throughout the gel matrix.
Specifications
- Catalogue Number: EC-828
- Composition: 241.6 g/L Acrylamide, 8.3 g/L Methylene Bisacrylamide, 7.5M Urea
- Cross-linker Ratio: 29:1 (Acrylamide:Bis)
- Maximum Monomer Concentration: 20%
- Certification: Certified Nuclease-Free (RNase/DNase)
- Storage: Room Temperature (20°C), protect from light
- Shelf Life: 12 months from date of manufacture
- Required Accessories: UreaGel Diluent (EC-840), UreaGel Buffer (EC-835), TEMED, and APS