TTE-Glycerol Tolerant Buffer (20 X): Eliminate Band Distortion
National Diagnostics TTE-Glycerol Tolerant Buffer (20 X) is a specialised electrophoresis reagent developed to solve a specific challenge in DNA sequencing: the distortion of bands caused by the presence of glycerol. When samples containing high concentrations of glycerol—common in many enzyme-based sequencing reactions—are run using standard TBE, the result is often “smiling” or blurred bands. TTE-Glycerol Tolerant Buffer eliminates these artefacts, providing crisp, horizontal band migration.
This buffer utilises a Taurine-based chemistry to replace the Borate found in standard TBE. The interaction between glycerol and borate typically creates a negatively charged complex that alters the local conductivity and mobility of the DNA. By substituting Borate with Taurine, the TTE system remains inert to glycerol, ensuring uniform migration across the entire gel and significantly improving the accuracy of automated and manual sequence reading.
Key Benefits:
- Eliminates Artifacts: Prevents “smiling” gels. Ensures straight, uniform band migration even when loading samples with high glycerol content.
- Improved Accuracy: Enhanced sequence resolution. Sharper bands lead to more accurate base calling and fewer ambiguous reads in sequencing workflows.
- Cooler Running: Reduced thermal stress. The Taurine-based system generates less heat at high voltages compared to TBE, protecting the gel matrix.
- High Compatibility: Optimised for automated systems. Fully compatible with various automated DNA sequencers and vertical PAGE formats.
Specifications
- Concentration: 20 X Liquid Concentrate
- Composition: Tris-Taurine-EDTA
- Storage: Store at room temperature (15-30°C)
- Shelf Life: 12 months
- Application: Glycerol-containing DNA sequencing samples, PAGE