PCRSizer 100bp DNA Ladder: Precision Sizing for PCR Analysis
The PCRSizer 100bp DNA Ladder is a high-performance molecular weight marker specifically engineered for the accurate sizing and quantification of double-stranded DNA fragments during agarose gel electrophoresis. Designed as an essential tool for routine molecular biology workflows, this ladder provides a reliable solution for researchers needing to confirm the size of PCR products and other small DNA fragments with confidence and precision.
This ladder comprises ten discrete, highly purified DNA fragments ranging from 100 bp to 1,000 bp in precise 100 bp increments. Each band is carefully prepared to ensure exceptional batch-to-batch consistency and sharp resolution. To facilitate easy orientation during gel analysis, the 500 bp band is provided at a higher intensity, serving as a clear reference point to allow for rapid, error-free identification of fragment sizes under UV transillumination.
Key Benefits:
- Defined 100bp Increments: Precise size estimation. Provides ten sharp, well-separated bands from 100 bp to 1,000 bp for detailed analysis of small DNA fragments.
- Enhanced 500bp Reference Band: Instant orientation. The increased intensity of the 500 bp fragment allows for rapid identification of band positions at a glance, even during extended runs.
- Optimised for PCR Confirmation: Tailored for routine use. Perfectly suited for verifying the success and size of a wide range of standard PCR amplicons.
- Superior Batch Consistency: Reliable results every time. Rigorous quality control and manufacturing standards ensure that migration patterns remain uniform across different lots.
- Convenient High-Yield Format: Efficient laboratory throughput. Provided in a format sufficient for 100 loads, supporting extensive laboratory testing and high-throughput workflows.
Technical Insights
Fragment Distribution and Visual Reference
The PCRSizer 100bp DNA Ladder is designed for intuitive visual analysis. By incorporating a high-intensity reference band at the 500 bp mark, researchers can quickly orient themselves and accurately determine the size of their samples without the need to count every band from the bottom. This feature is particularly beneficial for high-throughput screening where speed and accuracy are paramount.
Specifications
- Catalogue Numbers: L3-0006 (11400), L3-0007 (11415)
- Fragment Range: 100 bp – 1,000 bp
- Number of Bands: 10
- Increments: 100 bp
- Reference Band: 500 bp (Higher Intensity)
- Capacity: 100 Loads
- Application: DNA Gel Electrophoresis / PCR Product Sizing
- Storage: Store at -20°C for long-term stability