Fungi/Yeast Genomic DNA Isolation Kit – 27300: Rapid, High-Yield Extraction
The Fungi/Yeast Genomic DNA Isolation Kit (27300) provides a highly efficient, spin-column based solution designed specifically for the rapid preparation of high-quality genomic DNA from challenging microbiological samples. It is expertly formulated for the extraction of DNA from viable yeast cells, fungal spores or mycelium, as well as Gram-positive bacteria. Fungal and yeast cells are notorious for their robust, complex cell walls, making efficient lysis a critical bottleneck in sample preparation. This kit overcomes these challenges by combining powerful chemical detergents, heat treatment, and vigorous mechanical homogenisation using the provided Bead Tubes to ensure maximal cell disruption and DNA release.
Operating entirely without the need for toxic phenol or chloroform extractions, this streamlined protocol ensures researcher safety and significantly accelerates laboratory workflows, allowing for the complete processing of samples in under 30 minutes. For specific fungal strains or yeast species with exceptionally recalcitrant cell walls, the protocol seamlessly accommodates an optional lyticase treatment to further enhance yields. The resulting purified genomic DNA exhibits excellent structural integrity and is completely free from inhibitory contaminants, making it immediately ready for sensitive downstream analytical applications, including PCR, qPCR, Southern blotting, sequencing, and SNP analysis.
Key Benefits:
- Robust Cell Lysis: Mechanical and chemical disruption. Features specialised Bead Tubes for vigorous mechanical homogenisation, combined with optimised lysis buffers to effectively crack the toughest yeast and fungal cell walls.
- Rapid Processing: Under 30 minutes. The highly efficient spin-column format drastically reduces sample preparation time, enabling swift laboratory workflows without compromising nucleic acid quality.
- Exceptional Safety: Phenol and chloroform free. Avoid the occupational health hazards and costly waste disposal processes associated with traditional, highly toxic organic solvent extractions.
- High-Quality DNA Outputs: Ready for downstream assays. Delivers pristine genomic DNA that is fully compatible with sensitive enzymatic reactions, including quantitative PCR, RFLP, AFLP, and next-generation sequencing protocols.
- Broad Application Range: Isolate from diverse species. A versatile formulation that successfully extracts DNA from a wide variety of fungal and yeast species, as well as structurally demanding Gram-positive bacteria.
Technical Insights
Enhancing Yields with Optional Lyticase Treatment
While the combination of heat, strong detergents, and bead-beating provides highly effective lysis for the vast majority of routine samples, certain yeast and fungal species may exhibit uniquely thick cell walls that resist purely mechanical disruption. To ensure maximal recovery from these highly challenging samples, researchers can integrate a brief enzymatic pretreatment using lyticase prior to the mechanical homogenisation step. This targeted enzymatic digestion breaks down the structural polysaccharides of the cell wall, leaving the cells completely vulnerable to subsequent lysis and guaranteeing optimal genomic DNA yields.
Specifications
- Product Type: Spin-Column Genomic DNA Isolation Kit
- Maximum Column Binding Capacity: 50 µg
- Maximum Column Loading Volume: 650 µL
- Processing Time: ~30 minutes
- Maximum Starting Material (Fungi): 50 mg (wet weight)
- Maximum Starting Material (Yeast/Bacteria): 1 x 108 cfu’s (~0.5 – 1.0 mL culture)
- Format: 50 Preparations
- Storage: Room temperature (Stable for 1 year)