FastRunner DNA Ladder: High-Speed Molecular Weight Identification
The FastRunner DNA Ladder is a specialised molecular weight marker designed for the rapid and precise sizing of double-stranded DNA fragments between 50 bp and 2,000 bp. Engineered to meet the demands of high-throughput laboratories, this ladder addresses the common challenge of achieving clear, identifiable separation of small to mid-sized DNA fragments during fast agarose gel electrophoresis runs. It is an essential reagent for researchers requiring quick verification of PCR amplicons and restriction enzyme digestion products.
The ladder is composed of eight discrete, highly purified DNA fragments that provide a clear migration pattern across a broad range of agarose concentrations. To ensure ease of use and prevent interpretation errors, the 500 bp fragment is provided at a higher concentration, creating a distinct, high-intensity reference band. This strategic design allows for immediate orientation during gel documentation and ensures that researchers can accurately quantify and size their samples with minimal effort.
Key Benefits:
- Rapid Analysis: Designed for high-speed workflows. The optimised migration characteristics allow for quick sizing of PCR products and restriction digests without compromising band sharpness.
- Eight Sharp Fragments: Broad sizing range. Provides clear resolution from 50 bp to 2,000 bp, covering the most common experimental requirements for small to medium DNA fragment analysis.
- 500bp Reference Band: Intuitive orientation. The increased intensity of the 500 bp band facilitates rapid fragment identification and prevents miscounts during gel analysis.
- Batch-to-Batch Consistency: Reliable experimental reproducibility. Every batch is rigorously tested and prepared to ensure uniform performance and consistent band migration across different gel runs.
- High-Value 100 Load Format: Cost-effective throughput. Supplied in a format that supports 100 individual gel loads, making it ideal for routine use in busy molecular biology laboratories.
Technical Insights
Fragment Resolution and Identification
The FastRunner DNA Ladder is specifically balanced to provide sharp, non-distorted bands even during shorter electrophoresis run times. By utilising a wide range from 50 bp up to 2,000 bp, it provides a comprehensive solution for the majority of standard molecular biology screening applications. The distinct separation between the lower 50/100 bp bands and the upper 2,000 bp band ensures that both small and larger fragments can be accurately estimated on a single gel, saving time and reagents.
Specifications
- Catalogue Numbers: L3-0008 (12800), L3-0009 (12815)
- Fragment Range: 50 bp – 2,000 bp
- Number of Bands: 8
- Reference Band: 500 bp (High Intensity)
- Volume: 100 Loads
- Application: Fast Gel Electrophoresis / PCR Sizing / Restriction Digest Analysis
- Storage: Store at -20°C for long-term stability