FastGene® qFYR Real-Time qPCR System

FastGene® qFYR Real-Time qPCR System: High-Precision 96-Well Analysis

The FastGene® qFYR Real-Time qPCR System is a premier 96-well instrument designed to meet the rigorous demands of modern genomic analysis. Laboratories performing quantitative polymerase chain reaction (qPCR) require equipment that balances high throughput with absolute precision. This system delivers a versatile solution for gene expression analysis, genotyping, and pathogen detection, offering a standard plate format with advanced gradient functionality to support both routine screening and complex assay optimisation.

At the core of the qFYR is a patented optical detection architecture combining a high-quality Photomultiplier Tube (PMT) with a short focal length Fresnel lens. This design minimises the distance between the detector and the sample, significantly reducing signal loss and cross-talk for superior sensitivity. Complemented by a unique hollowed-out thermal block with state-of-the-art Peltier components, the system ensures exceptional temperature uniformity (± 0.2 °C) and rapid heating/cooling rates, guaranteeing reproducible data across all 96 wells.

Key Benefits:

• Multiplex Capability: Advanced 4+1 channel architecture. Features four distinct colour channels plus a secondary FAM/SYBR channel for fast dual-mode melt curve analysis, enabling efficient multiplexing.
• Superior Thermal Control: Precision Peltier technology. Achieves heating and cooling rates of up to 6 °C/s with temperature uniformity of ± 0.2 °C, ensuring consistent reaction kinetics across the plate.
• Enhanced Sensitivity: Patented optical design. The short focal distance reduces cross-talk and signal loss, providing outstanding homogeneity and detection limits for low-abundance targets.
• Versatile Applications: Comprehensive analysis suite. Fully optimised for gene expression, genotyping, High Resolution Melt (HRM), and pathogen detection with intuitive, user-friendly software included.

Technical Insights

High Sensitivity Optical Design

The FastGene® qFYR utilises a unique, patented optical detection system. By combining a high-quality PMT (photomultiplier tube) with a Fresnel lens possessing a short focal length, the distance from detector to sample is minimised. This engineering reduces signal loss and cross-talk between samples, generally improving signal sensitivity for critical measurements.

Superior Thermal Cycler Performance

The system delivers excellent temperature control and uniformity over the entire 96-well plate. The unique hollowed-out thermal block design reduces overall weight, facilitating rapid heating and cooling rates (up to 6 °C per second) for fast qPCR protocols. State-of-the-art Peltier components ensure the highest reaction quality and performance stability.

The Powerful User Software Has It All

The analysis software (FastGene® qFYR Analysis Studio) impresses with its particularly simple operation. The menu is clearly structured and intuitively arranged, responding to the specific needs of users for various experimental setups. Personalized settings can be adjusted with ease, while integrated analysis algorithms allow critical steps—such as baseline subtraction and Cq value threshold calculation—to be performed automatically.

Absolute and relative quantification of nucleic acids is fully automated within the platform. The software enables users to save predefined analysis protocols for the auto-exporting of run data into a format of choice, including Excel, PDF, and txt.

• Clearly structured and user-friendly
• Intuitive navigation
• Customisable settings
• Automatic analysis with integrated algorithms
• Modules for multiple applications
• Modern UI design

Specifications

• Block Format: 96-well block with gradient function
• Channels: 4 standard colour channels + 1 extra FAM/SYBR channel
• Channel Configuration:
  • Channel 1: FAM/SYBR Green
  • Channel 2: VIC/JOE/HEX/TET
  • Channel 3: ROX/Texas Red
  • Channel 4: Cy5
  • Channel 5: FAM/SYBR Green (Fast Mode)
• Temperature Precision: ± 0.2 °C
• Ramp Rate: Up to 6 °C/s (Heating/Cooling)
• Scan Time: 8 seconds (single plate scan)
• Software: FastGene® qFYR Analysis Studio (Included)
• Applications: Gene expression, Absolute/Relative quantification, Genotyping, Pathogen detection, miRNA analysis, Protein stability screening, HRM