P8-0043
FastGene® HiFi HS (Hot Start) Polymerase 2x Master Mix

FastGene® HiFi HS polymerase has a significantly improved DNA binding activity and processivity compared to standard Pfu.This results in faster extension, higher yields and the robust amplification of difficult targets.
P8-0043
Catalogue NumberDescriptionPack SizePrice
P8-0043FastGene® HiFi HS (Hot Start) Polymerase 2x Master Mix100 Reactions£203.00
The high fidelity of the FastGene® HiFi HS (Hot Start) Polymerase is based on the improved 3`-5` exonuclease activity, which significantly reduces the error rate of the enzyme and makes it around 100 times more accurate than a Taq DNA polymerase. This is ideal for applications where high fidelity is essential, such as sequencing, cloning and site-directed mutagenesis.
  • Reliable high fidelity polymerase – 100x higher fidelity than Taq DNA polymerase
  • Special hot start technology for maximum sensitivity and specificity (see below)
  • Superior performance under difficult conditions, e.g., AT or GC-rich sequences and long templates (up to 17.5 kb)
  • Perfect for mulitplex-PCRs and applications where high accuracy is required (e.g., cloning, site-directed mutagenesis, sequencing)
  • Ready-to-use cocktail with all components neccessary for a wide range of targets and fragment sizes
  • 3’-5’ exonuclease and proof-reading activity
  • Easy experimental set-up at room temperature
  • Stability of the reaction mixture for up to 24 hours before and after the PCR run

Details

High fidelity is the key

The high fidelity of the FastGene® HiFi HS (Hot Start) Polymerase is based on the improved 3`-5` exonuclease activity, which significantly reduces the error rate of the enzyme and makes it around 100 times more accurate than a Taq DNA polymerase. This is ideal for applications where high fidelity is essential, such as sequencing, cloning and site-directed mutagenesis.  

Superior hot start technology

The FastGene® HiFi HS Polymerase uses a special technology with an aptamer-like molecule that gives the polymerase hot start activity that is different from the antibody-mediated hot start normally used. The aptamer-like molecule reversibly binds and inhibits the 3′-5′ exonuclease and 5′-3′ polymerase activities of the enzyme at temperatures below 40°C. This sophisticated hot start molecule prevents primer dimer formation and non-specific amplification. It therefore maximises the sensitivity and specificity of your PCR and allows easy experimental set-up at room temperature.  

Save time with the Master Mix

The FastGene® HiFi Polymerase is provided in a 2x Master Mix, which significantly speeds up the preparation of a PCR. It contains an advanced buffering system with dNTPs, Mg2+, reaction enhancers and the polymerase enzyme.  

Product applications

The FastGene® HiFi HS 2x Master Mix (LS36) is ideally suited for:
  • Accurate Cloning
  • Sequencing
  • Site-directed mutagenesis
  • High fidelity PCR
  • Long range PCR
  • Any standard PCR application for which a hot start formulation of a high-quality thermostable DNA polymerase is require

Challenging PCR? Perfect for FastGene® HiFi Polymerase!

The FastGene® HiFi Polymerase is the high-fidelity enzyme for precise PCR amplifications. The enzyme was engineered in a way that it can amplify particularly long templates (up to 17.5 kb) with a high sequence accuracy. Furthermore, it shows a significant improvement in extension times (10-30 sec per kb), while generating high yields, even with difficult templates.  

Amplify long fragments

Figure 1: The FastGene® HiFi Polymerase is capable of amplifying a long range of fragments, even up to 17500 bp. (The image was generated with MIDORIGreen Xtra safe DNA dye and FastGene® FAS-DIGI PRO gel documentation system.)  

Deal with tough templates

Figure 2: Templates with varying GC-content, ranging from ~30 % – 80 % can be successfully amplified with the FastGene® HiFi Polymerase. (The image was generated with MIDORIGreen Xtra safe DNA dye and FastGene® FAS-DIGI PRO gel documentation system.)  

Perform easy multiplexing

  Figure 3: 6 phage DNA fragments, 4 mouse DNA fragments and a combination of both were successfully amplified with the FastGene® HiFi Polymerase in a multiplex PCR. (The image was generated with MIDORIGreen Xtra safe DNA dye and FastGene® FAS-DIGI PRO gel documentation system.)    

Specification

Documentation

Protocol
P8-0043 Protocol
Catalogue NumberDescriptionPack SizePrice
P8-0030FastGene® Taq DNA Polymerase500 Reactions£53.5
P8-0042FastGene® Optima HotStart Ready Mix500 Reactions£191
T3-0176SensoQuest Labcycler 48s Including Silver 48 x 0.2ml BlockEach£3834
T3-0180FastGene Ultra Cycler Gradient Thermal Cycler Including 96 x 0.2ml BlockEach£4498