DNA Spin Column & 2.0ml Collection Tubes: Universal Silica-Based Purification
The Geneflow DNA Spin Column and 2.0ml Collection Tube system provides a high-performance, cost-effective solution for researchers looking to maximise their laboratory resources. These columns are engineered with premium silica membranes that offer universal compatibility with almost all silica-based DNA extraction, PCR purification, and gel extraction protocols. By providing a reliable alternative to kit-specific hardware, these columns allow laboratories to utilise surplus buffers from various commercial kits, significantly reducing waste and overhead costs without compromising on yield or purity.
At the core of the system is a high-grade silica matrix designed for the “bind-wash-elute” workflow. Under high chaotropic salt conditions, the membrane selectively adsorbs DNA while allowing proteins, salts, and other inhibitors to be washed away into the included 2.0ml collection tubes. Whether you are performing routine PCR clean-up, isolating genomic DNA from complex tissues, or conducting small-scale plasmid preparations from midi-prep buffer sets, the precise capillary structure of the membrane ensures consistent flow rates and high binding capacity across every prep.
Key Benefits:
- Universal Kit Compatibility: Seamlessly integrates with major brands. Fully compatible with buffers and protocols from Geneflow, Qiagen, Thermo Fisher, and other leading suppliers for PCR purification, gel extraction, and DNA isolation.
- Exceptional Binding Capacity: High-yield nucleic acid recovery. The multi-layer silica membrane is optimised to bind up to 30 µg of DNA, making it suitable for both high-concentration plasmid preps and delicate genomic extractions.
- Versatile Application Range: One column for every workflow. Proven performance across PCR clean-up, enzymatic reaction stripping, and even as a mini-scale alternative for processing fractions from larger midi-prep workflows.
- Robust Labware Design: Engineered for high-speed centrifugation. The 2.0ml collection tubes are manufactured from medical-grade polypropylene, designed to withstand centrifugal forces up to 16,000 x g without cracking or leaking.
- Certified Nuclease-Free: Maintains sample integrity. Every batch is guaranteed to be DNase and RNase-free, ensuring that your purified DNA remains stable and ready for sensitive downstream applications like NGS or qPCR.
Technical Insights
The Bind-Wash-Elute Process
The universality of these columns stems from the standardised silica-DNA interaction. By maintaining optimal pH and salt concentrations provided by your existing kit buffers, the membrane facilitates rapid dehydration of the DNA phosphodiester backbone, allowing it to bind tightly to the silica surface. This process effectively separates the DNA from PCR primers, dNTPs, and enzymes in minutes.
Specifications
- Membrane Type: High-purity Silica Membrane
- Reservoir Volume: 800 µl
- Fragment Size Range: 50 bp – 30 kb
- Purity Ratio (A260/280): 1.8 – 2.0
- Applications: Genomic DNA Extraction, PCR Clean-up, Gel Extraction, Plasmid Miniprep
- Compatibility: Compatible with all silica based DNA Extraction systems. e.g. Qiagen, Invitrogen, and Promega buffer systems