P4-0434
Adenovirus Purification Kit – 67600

Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger.  Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. Adenoviral vectors purified in this manner are highly active for use in transduction experiments.
P4-0409 Image
Catalogue NumberDescriptionPack SizePrice
P4-0434Adenovirus Purification Kit – 6760015 Preps£1,008.00
 
  • Adenovirus Purification from any input – cell fraction or media fraction
  • Rapid purification within 2 to 4.5 hours
  • No specialized equipment needed (ultracentrifuge not required)
  • Purify adenovirus cell culture supernatant from 1 mL to 33.5 mL input per prep
  • Purify adenovirus cell pellet from 1 mL of input per prep
  • Up to 25X sample concentration
Adenoviral vectors are useful tools for both in vitro and in vivo gene transfer, and oncolytic viruses based on adenovirus are highly promising for cancer treatment. Norgen’s Adenovirus Purification Kit provides a fast and simple procedure for concentrating and purifying adenoviral vectors from cell lysate and cell culture media. Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger.  Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. Adenoviral vectors purified in this manner are highly active for use in transduction experiments. Norgen’s Adenovirus Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit.

Specification

Specification
Resin Binding Capacity (total per kit); At least 3x1011 adenovirus particles as determined by qPCR At least 3x108 transducing units as determined by transduction assay Input type; Cells, media Input volume (Supernatant); 1 - 33.5 mL SN per prep (500 mL SN in total) Input Volume (Cell pellet); 1 mL cell pellet per prep (15 mL in total) Minimum elution volume; 1.3 mL per prep Time to complete purification; 2.5 to 4.5 hours with 1 hour hands on time In vivo transduction; Yes

Documentation

Protocol
P4-0434 Protocol 67600
Catalogue NumberDescriptionPack SizePrice
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E7-0264TransIT-VirusGEN® Transfection Reagent – MIR 67040.75ml£429
P4-0409AAV Purification Kit – 6610015 Preps£1040
P4-0202AAV Purification Mini Kit – 6320020 Preps£434
P4-0201AAV Purification Midi Kit – 633004-8 Preps£435
P4-0432AAV Purification Maxi Slurry Kit – 632501-10 Preps£1285