TBST (10X): High-Purity Western Blotting Wash and Incubation Buffer
TBST (Tris-Buffered Saline with Tween-20) is a fundamental reagent in molecular biology, serving as the primary wash and incubation buffer for Western blotting and other immunoassays. It is specifically designed to maintain pH stability and provide the necessary ionic strength to reduce non-specific background interference, ensuring that antibody-antigen interactions remain specific and detectable. By providing a reliable environment for both membrane washing and antibody dilution, TBST is essential for producing high-quality, reproducible blots with clear signal definition.
This 10X concentrated formulation is manufactured using ultra-pure reagents and subjected to stringent quality control protocols to guarantee batch-to-batch consistency. Upon dilution to a 1X working concentration, the buffer yields a precise composition of 25 mM Tris, 137 mM sodium chloride, 2.7 mM potassium chloride, and 0.05% Tween-20. The inclusion of Tween-20, a non-ionic detergent, is critical for preventing non-specific hydrophobic interactions between the primary/secondary antibodies and the membrane, thereby significantly improving the signal-to-noise ratio in protein detection workflows.
Key Benefits:
- Ultra-Pure Formulation: Ensures experimental reproducibility. Stringent quality control and high-purity components prevent contaminants from interfering with sensitive protein detection assays.
- Efficient 10X Concentrate: Streamlines laboratory workflows. The concentrated format reduces storage space requirements and allows for the rapid preparation of large volumes of working buffer.
- Optimised Signal Clarity: Maximises signal-to-noise ratios. The balanced detergent and salt concentrations effectively remove unbound antibodies without compromising the integrity of target protein bands.
- Versatile Application: Supports multiple immunoassay stages. Ideal for use in both the washing stages and as a diluent for primary and secondary antibodies.
Technical Insights
The standard 1X working solution provides a stable physiological pH and ionic environment. The specific salt concentration (NaCl and KCl) maintains the osmotic balance required for protein stability on the membrane, while the Tris component provides robust buffering capacity. The 0.05% Tween-20 concentration is carefully calibrated to be sufficient for blocking non-specific sites without being aggressive enough to strip bound proteins or inhibit enzymatic detection markers.
Specifications
- Catalogue Number: B9-0076
- Concentration: 10X Liquid Concentrate
- 1X Buffer Composition: 25 mM Tris, 137 mM NaCl, 2.7 mM KCl, 0.05% Tween-20
- Applications: Western Blotting (Wash/Incubation), ELISA, Immunohistochemistry
- Storage: Room Temperature (ensure the seal is maintained to prevent evaporation)
- Quality Grade: Ultra-pure, molecular biology grade