TransIT-siQUEST® Transfection Reagent: Alternative Broad-Spectrum siRNA Delivery
The TransIT-siQUEST® Transfection Reagent is a broad-spectrum formulation designed to enable high-efficiency siRNA delivery and robust knockdown of target gene expression in a wide variety of cell types, including primary cells. Recognising that different cell lines respond differently to specific transfection chemistries, TransIT-siQUEST offers a unique formulation distinct from TransIT-TKO® and TransIT-X2®. This provides researchers with an alternative high-performance solution to ensure successful gene silencing even in cell types that may be refractory to other reagents.
Engineered for experimental flexibility and ease of use, TransIT-siQUEST maintains high efficiency in the presence of serum, eliminating the need for medium changes or serum-free incubation steps. The protocol is streamlined to minimise cellular manipulation, ensuring that cell health and viability are preserved during the transfection process. Whether used for routine gene knockdown or high-throughput screening, this reagent delivers consistent and reproducible results.
Key Benefits:
- distinct Formulation: Alternative Chemistry. Provides a unique broad-spectrum formulation that complements TransIT-TKO and TransIT-X2, offering an alternative solution for difficult-to-transfect cells.
- High Efficiency: Robust Knockdown. Delivers high-efficiency gene silencing in a wide range of cell lines, including adherent and suspension cells.
- Streamlined Workflow: Serum Compatible. Performed directly in complete growth medium; no post-transfection medium change is required, saving time and reducing contamination risk.
- Physiological Relevance: Low Toxicity. Gentle formulation maintains cell density and morphology, ensuring that experimental data reflects gene knockdown rather than reagent-induced stress.
- Versatile Application: Sequential Transfection. Compatible with sequential transfection protocols for plasmid DNA and siRNA delivery when used alongside TransIT-LT1 or TransIT-2020.
Technical Insights
Protocol Optimisation
To maximise knockdown efficiency, the TransIT-siQUEST protocol allows for optimisation based on the specific cell type. For a standard 24-well plate, the recommended starting condition is 1.5 µl of reagent per well. However, researchers can fine-tune performance by titrating the reagent volume between 0.5 µl and 3 µl. The recommended starting siRNA concentration is 25 nM, with a typical working range of 10–50 nM.
Specifications
- Product Type: Transfection Reagent (siRNA)
- Storage Conditions: Store tightly capped at 4°C. Warm to room temperature and vortex gently before use.
- Shelf Life: 1 year from the date of purchase when properly stored and handled.
- Reagent Compatibility: Compatible with serum-containing medium (complete growth medium).
- Antibiotic Compatibility: Transfection complexes can be added to cells grown in medium containing low levels of antibiotics (0.1–1X).
- Ordering Information:
- E7-0116: 1 x 0.4 ml (MIR 2114)
- E7-0114: 1 x 1.5 ml (MIR 2110)
- E7-0118: 5 x 1.5 ml (MIR 2115)
- E7-0120: 10 x 1.5 ml (MIR 2116)