TransIT-TKO® Transfection Reagent: The Original Broad-Spectrum siRNA Solution
The TransIT-TKO® Transfection Reagent is a broad-spectrum formulation designed to enable high-efficiency siRNA delivery and robust knockdown of target gene expression across a wide variety of cell types, including challenging primary cells. Introduced as the first commercially available siRNA transfection reagent, TransIT-TKO has established a proven track record in the field of RNA interference (RNAi). It provides researchers with a reliable tool for investigating gene function through effective post-transcriptional silencing.
Beyond standard siRNA delivery, TransIT-TKO is highly versatile, facilitating the co-transfection of plasmid DNA and siRNA for simultaneous gene expression and knockdown studies. The reagent is engineered for ease of use and cellular compatibility; transfections can be performed directly in serum-containing medium without the need for post-transfection medium changes. This streamlined workflow minimises cellular stress and handling time, ensuring that experimental results are both reproducible and physiologically relevant.
Key Benefits:
- Proven Performance: First-in-Class. The first commercially available siRNA transfection reagent, validated for high-efficiency knockdown across diverse cell lines.
- Broad Compatibility: Primary & Immortalised Cells. Effectively delivers siRNA into a wide range of cell types, including difficult-to-transfect primary cells.
- Versatile Application: Co-Transfection Capable. Supports the simultaneous delivery of plasmid DNA and siRNA, enabling complex experimental designs.
- Streamlined Workflow: Serum Compatible. No medium change is required after transfection; complexes can be added directly to cells in complete growth medium.
- Low Toxicity: Cellular Integrity. Gentle formulation preserves cell health, ensuring that observed phenotypes are due to gene knockdown rather than reagent toxicity.
Technical Insights
Protocol Optimisation
To achieve optimal knockdown efficiency, the TransIT-TKO protocol offers flexible optimisation guidelines. For a standard 24-well plate, the recommended starting condition is 2.5 µl of reagent per well with a final siRNA concentration of 25 nM. Researchers can further optimise delivery by testing reagent volumes between 1–4 µl. When performing co-transfection, it is recommended to synchronise the formation of DNA and siRNA complexes before adding them to the cells.
Specifications
- Product Type: Transfection Reagent (siRNA)
- Storage Conditions: Store tightly capped at 4°C. Warm to room temperature and vortex gently before use.
- Shelf Life: 1 year from the date of purchase when stored properly.
- Reagent Compatibility: Compatible with serum-containing medium (complete growth medium).
- Antibiotic Compatibility: Transfection complexes can be added to cells grown in medium containing low levels of antibiotics (0.1–1X).
- Ordering Information:
- E7-0108: 1 x 0.4 ml (MIR 2154)
- E7-0106: 1 x 1.5 ml (MIR 2150)
- E7-0110: 5 x 1.5 ml (MIR 2155)
- E7-0112: 10 x 1.5 ml (MIR 2156)