TransIT®-Lenti Transfection Reagent: Optimised for Lentivirus Production
TransIT®-Lenti Transfection Reagent is a specialised formulation designed to maximise the production of recombinant lentivirus in HEK 293T cells. Generating high-titer viral stocks is often a bottleneck in gene delivery workflows; TransIT-Lenti addresses this by enhancing the delivery of packaging and transfer vectors, resulting in functional titers that significantly outperform traditional calcium phosphate or liposomal methods. This reagent is an essential tool for researchers relying on lentiviral transduction for stable gene expression or CRISPR screening.
The protocol is engineered for simplicity and consistency. It is fully animal-origin free and compatible with serum-containing media, meaning no medium changes are required post-transfection. This single-harvest workflow not only saves time but also reduces the physical stress on producer cells, leading to higher viability and cleaner viral supernatants. TransIT-Lenti scales easily from small-scale screening to larger production formats.
Key Benefits:
- High Titers: Superior Production. Yields functional lentiviral titers that are consistently higher than those achieved with Calcium Phosphate or Lipofectamine® reagents.
- Optimised Formulation: Lenti Specific. Specifically developed to enhance the co-transfection of packaging plasmids and transfer vectors in HEK 293T cells.
- Regulatory Friendly: Animal-Origin Free. Suitable for use in regulated environments and biotherapeutic development.
- Streamlined Workflow: No Media Changes. Perform transfections in complete growth medium; eliminates wash steps and media replacements.
- Flexible Scaling: Versatile Use. Effective for both small-scale research and larger volume viral production.
Technical Insights
Protocol Optimisation
To maximise viral titers, the TransIT-Lenti protocol uses a specific reagent-to-DNA ratio. The recommended starting condition is 3 µl of TransIT-Lenti Reagent per 1 µg of total DNA (transfer vector + packaging mix). Complexes are formed in serum-free medium (e.g., Opti-MEM®) and incubated for 10 minutes before being added to cells. Viral supernatants are typically harvested 48 hours post-transfection.
Specifications
- Product Type: Transfection Reagent (Lentivirus Specific)
- Storage Conditions: Store at -20°C. Warm to room temperature and vortex gently before use.
- Shelf Life: 1 year from the date of purchase when stored properly.
- Reagent Compatibility: Compatible with serum-containing medium.
- Ordering Information:
- E7-0222: 1 x 0.3 ml (MIR 6603)
- E7-0224: 1 x 0.75 ml (MIR 6604)
- E7-0226: 1 x 1.5 ml (MIR 6600)
- E7-0228: 5 x 1.5 ml (MIR 6605)
- E7-0230: 10 x 1.5 ml (MIR 6606)