Stool Nucleic Acid Isolation Kit: High-Yield Microbiome and Pathogen Recovery
The Stool Nucleic Acid Isolation Kit is a comprehensive solution designed to address the inherent challenges of extracting high-quality genetic material from faecal samples. Stool is a notoriously difficult matrix, often containing high concentrations of PCR inhibitors such as humic acids, bile salts, and complex polysaccharides that can compromise downstream assays. This kit provides a streamlined workflow to isolate both genomic DNA and total RNA from human or animal stool, ensuring total representation of the microbial community and host cells.
The system utilises advanced silica-based spin column technology in conjunction with a robust chemical and mechanical lysis protocol. By employing specialized Inhibitor Removal chemistry, the kit selectively precipitates and eliminates contaminating substances without the need for toxic organic extractions like phenol or chloroform. The resulting purified nucleic acids are of exceptional purity and integrity, ready for immediate use in sensitive molecular biology applications.
Key Benefits:
- Universal Extraction: Co-purify DNA and RNA. Simultaneously isolates both genomic DNA and total RNA from a single sample for comprehensive metagenomic and transcriptomic profiling.
- Superior Inhibitor Removal: Highly purified templates. Effectively eliminates humic acids and polyphenols, ensuring consistent performance in qPCR, NGS, and enzymatic reactions.
- Optimised Lysis: Efficient microbial recovery. Validated for the lysis of tough-to-disrupt organisms, including Gram-positive bacteria and yeast, ensuring an unbiased view of the stool microbiome.
- Rapid Workflow: Time-efficient processing. The simple spin-column format allows for the completion of the entire purification process in under 45 minutes.
Technical Insights
The kit has been rigorously tested to ensure that the ratio of A260/A280 remains consistently between 1.8 and 2.1, indicating high-purity nucleic acids free from protein contamination. Furthermore, the inclusion of a proprietary bead-beating or chemical lysis step (depending on sample type) ensures the breakdown of complex cellular structures without shearing the genetic material.
Specifications
- Product Code: 45600
- Sample Type: Fresh or frozen stool samples (Human or Animal)
- Sample Input: Up to 200 mg of solid stool or 200 µl of liquid stool
- Elution Volume: 50 µl – 100 µl
- Storage Conditions: Room Temperature (15-25°C)
- Shelf Life: 12-18 months when stored correctly
- Downstream Compatibility: qPCR, RT-qPCR, NGS, Microarrays, and Restriction Enzyme Digestion