Preserved Blood RNA Purification Kit: High-Integrity RNA Isolation from Tempus™ Tubes
The Preserved Blood RNA Purification Kit I is an advanced molecular tool designed for the rapid isolation and purification of total RNA from blood samples collected in Tempus™ Blood RNA Tubes. Recognising the critical need for preserving RNA integrity in clinical and research settings, this kit provides a streamlined solution that captures the complete transcriptome while effectively removing cellular debris and proteins that can interfere with sensitive assays. It is an essential partner for laboratories performing high-resolution gene expression analysis where sample stability is paramount.
Utilising specialised spin column chromatography, the system employs a proprietary resin as the separation matrix. This technology ensures superior affinity for the full spectrum of RNA molecules, from large messenger RNA (mRNA) and ribosomal RNA (rRNA) to small regulatory species like microRNA (miRNA) and small interfering RNA (siRNA). The process is entirely phenol-free and chloroform-free, delivering high-purity nucleic acids with exceptional linearity and sensitivity across all size ranges, ensuring the highest integrity for downstream genomic applications.
Key Benefits:
- Comprehensive Transcriptome Capture: Isolate the full RNA spectrum. Ensures the recovery of both large and small RNA molecules, including miRNA and siRNA, providing a complete and unbiased picture of gene expression.
- Superior Sample Purity: Generate inhibitor-free results. The proprietary resin technology aggressively removes proteins and other cellular contaminants, resulting in high-integrity RNA suitable for the most demanding applications.
- Enhanced Safety and Speed: No hazardous chemical extractions. The rapid protocol eliminates the need for phenol or chloroform, reducing processing time to just 20 minutes and improving laboratory safety.
- Optimised for Tempus™ Tubes: Directly compatible workflow. Specifically engineered to work seamlessly with Tempus™ Blood RNA Tubes, ensuring efficient lysis and maximal recovery from preserved blood samples.
- Excellent Linearity: Consistent and sensitive detection. The unique binding properties of the resin matrix provide better sensitivity for low-abundance transcripts compared to traditional silica-based methods.
Technical Insights
Isolation of High Quality Total RNA
Analysis via Agilent Bioanalyzer demonstrates the isolation of high-integrity RNA from blood samples. The electropherograms typically show clearly visible 18S and 28S rRNA fragments with RNA Integrity Number (RIN) scores reaching 9.0, confirming that the purification process maintains the molecular weight of the transcripts without degradation.
Detection of Diversity in RNA Species
The system is validated for the recovery of varied RNA sizes. RT-qPCR results confirm the successful amplification of large transcripts (such as Beta-Actin), small ribosomal RNA (5S rRNA), and regulatory microRNA (miR-21) from the same elution, proving the kit’s versatility for both mRNA and miRNA profiling.
Specifications
- Catalogue Number: P4-0129
- Sample Type: Blood preserved in Tempus™ Blood RNA Tubes
- Pack Size: 50 Preparations
- RNA Size Range: All sizes (mRNA, rRNA, miRNA, siRNA, and viral RNA)
- Maximum Binding Capacity: 50 µg per column
- Average Yield: 5 – 25 µg per 3 mL preserved human blood
- Time to Complete: 20 – 30 minutes for 10 samples
- Storage Conditions: All solutions stable at Room Temperature (15-25°C)
- Shelf Life: 2 years from date of shipment