PCRRanger 100bp DNA Ladder: Versatile Resolution for Routine PCR Analysis
The PCRRanger 100bp DNA Ladder is a robust molecular weight marker designed for the precise sizing and semi-quantification of double-stranded DNA fragments. As a staple for routine laboratory workflows, this ladder provides a broad sizing range that is particularly well-suited for the analysis of PCR amplicons, restriction digests, and genomic DNA screening. It addresses the need for a versatile reference that maintains clarity across a wide range of fragment lengths without sacrificing resolution in the critical lower-weight regions.
This ladder is composed of 13 highly purified DNA bands ranging from 100 bp to 3,000 bp. To ensure efficient interpretation during electrophoresis, the formulation includes two reinforced reference bands at 500 bp and 1,500 bp, providing instant orientation even during extended gel runs. The PCRRanger is supplied in a ready-to-use format, containing high-density loading buffer and dual tracking dyes (bromophenol blue and xylene cyanol FF) to monitor migration progress in real-time, reducing preparation steps and ensuring consistent results.
Key Benefits:
- Enhanced Navigation: Dual high-intensity reference bands. Increased DNA mass at 500 bp and 1,500 bp allows for rapid and unambiguous identification of fragment sizes.
- Broad Analytical Range: 13 discrete bands. Covers a wide spectrum from 100 bp to 3 kb, making it suitable for almost all standard PCR applications.
- Optimised Visualisation: Dual-colour tracking. Includes two tracking dyes that migrate at different rates, allowing researchers to monitor the migration of both small and large fragments effectively.
- Maximum Convenience: Ready-to-load formulation. Eliminates the need for manual mixing or the addition of dyes, streamlining the transition from PCR thermal cycler to gel tank.
Technical Insights
Band Distribution and Mass
The PCRRanger (11300/11315) features fragments at: 100, 200, 300, 400, 500 (Ref), 600, 700, 800, 900, 1,000, 1,500 (Ref), 2,000, and 3,000 bp. This logarithmic distribution ensures that the ladder remains effective on 1.0% to 2.0% agarose gels, providing sharp resolution for both mid-range and small fragments.
Specifications
- Fragment Range: 100 bp – 3,000 bp
- Number of Bands: 13
- Reference Bands: 500 bp and 1,500 bp (High intensity)
- Tracking Dyes: Bromophenol Blue and Xylene Cyanol FF
- Recommended Loading: 5 µl per lane
- Total Loads: 100 (11300) or Bulk options (11315)
- Storage: Store at 4°C for frequent use; -20°C for long-term stability