Ingenio® Electroporation Solution: Universal High-Efficiency Reagent
The Ingenio® Electroporation Solution offers a high-performance, universal alternative for researchers seeking to optimise gene delivery while reducing consumable costs. Designed to replace proprietary, instrument-specific buffers, this solution facilitates the efficient delivery of DNA and siRNA into hard-to-transfect cell types, including primary cells and stem cells. By providing a stable, optimised environment for permeabilisation, Ingenio® minimises the high cellular toxicity often associated with electroporation, preserving cell viability without sacrificing transfection efficiency.
This standalone solution provides exceptional versatility for laboratories utilising multiple platforms. It is formulated to work seamlessly with “open system” electroporators as well as standard instruments such as the Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser®, and Harvard-BTX® systems. Whether your protocol requires exponential decay or square wave pulses, Ingenio® allows you to standardise your workflow with a single, cost-effective reagent.
Key Benefits:
- Universal Compatibility: One buffer for all instruments. Validated for use with the Amaxa Nucleofector (I, II/2B), Bio-Rad Gene Pulser, and BTX electroporators, eliminating the need for multiple device-specific kits.
- High Efficiency Delivery: Superior gene expression. Delivers nucleic acids effectively into difficult targets such as Jurkat E6-1, human keratinocytes, and varying stem cell lines.
- Low Cellular Toxicity: Maximise cell survival. The formulation is specifically developed to reduce cell death post-electroporation, ensuring a higher yield of viable, expressing cells.
- Cost-Effective Format: Significant savings. Available as a standalone solution, allowing you to use your own cuvettes or purchase accessories separately to lower running costs compared to complete kits.
- Flexible Pulse Support: Broad protocol application. Compatible with both exponential decay and square wave pulse forms, supporting diverse experimental requirements.
Technical Insights
The Ingenio® Electroporation Solution is designed for broad spectrum application. One millilitre of solution is sufficient for approximately 4 electroporations in 0.4 cm cuvettes or 10 electroporations in 0.2 cm cuvettes, making it a highly efficient choice for scaling experiments.
Specifications
- Storage Conditions: Store at 4°C.
- Shelf Life: Guaranteed for 1 year from the date of purchase.
- Instrument Compatibility: Mirus Bio Ingenio® EZporator®, Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser®, Harvard-BTX®.
- Pulse Compatibility: Exponential decay and square wave.
- Ordering Information:
- Geneflow E7-0516 (MIR 50111): 1 x 6.25 ml
- Geneflow E7-0518 (MIR 50114): 1 x 12.5 ml
- Geneflow E7-0520 (MIR 50117): 25 ml (Supplied as 2 x 12.5 ml)