FFPE RNA Purification Kit: Reliable Isolation from Archival Tissues
Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples are an invaluable resource for clinical research and retrospective studies. However, they present significant hurdles for RNA isolation due to chemical cross-linking and nucleic acid degradation caused by the fixation process. The FFPE RNA Purification Kit provides a streamlined solution, overcoming the inhibitory effects of formalin to deliver high-quality, intact RNA suitable for sensitive downstream applications such as RT-qPCR, microarrays, and RNA-Seq.
This system utilises a specialised, non-toxic deparaffinisation buffer, eliminating the need for hazardous organic solvents like xylene or histolene. Following tissue digestion with Proteinase K, the optimised buffer conditions facilitate the effective reversal of formalin-induced cross-links. The RNA is then selectively bound to a silica-based spin column under high-salt conditions, washed to remove proteins and contaminants, and eluted in a concentrated, high-purity form ready for immediate use.
Key Benefits:
- Solvent-Free Deparaffinisation: Safe and rapid processing. Utilises a non-toxic buffer instead of xylene, reducing health risks and simplifying waste disposal workflows within the laboratory.
- Optimised Cross-link Reversal: Superior RNA Integrity. Specifically formulated lysis buffers ensure the maximum recovery of even fragmented RNA while effectively removing genomic DNA contamination.
- Streamlined Protocol: Results in under 60 minutes. The simplified spin-column format significantly reduces hands-on time compared to traditional phenol-chloroform extraction methods.
- High Binding Capacity: Scalable yields. The silica membrane is engineered to bind up to 50 µg of RNA, making it suitable for varying sample sizes including small needle biopsies.
Technical Insights
The efficiency of the FFPE RNA Purification Kit is demonstrated through the successful removal of paraffin and the subsequent release of nucleic acids from the protein-nucleic acid matrix. By avoiding harsh solvents, the kit preserves the biological integrity of the RNA, leading to improved performance in quantitative PCR and next-generation sequencing.
Specifications
- Catalogue No.: P4-0072
- Sample Type: FFPE tissue sections (up to 10 mg or 7 sections of 10 µm thickness)
- Binding Capacity: 50 µg per column
- Elution Volume: 20–50 µL
- Preparation Time: Approximately 60 minutes (excluding lysis time)
- Storage: Main kit components at room temperature (15–25°C); Proteinase K at -20°C
- Downstream Compatibility: RT-qPCR, RNA-Seq, Microarrays, Northern Blotting