FastGene DNAreleasy Advance: Rapid DNA Release for Direct PCR
The FastGene DNAreleasy Advance replaces time-consuming DNA extraction and purification steps with a rapid, single-tube lysis protocol. Designed for researchers requiring immediate template preparation, this reagent delivers PCR-ready DNA in as little as 15 minutes. It is an ideal solution for high-throughput genotyping and screening applications where speed and workflow efficiency are critical.
The reagent utilises a complex formulation that effectively lyses cells and tissue, releasing genomic DNA into the supernatant while maintaining its integrity for amplification. Unlike traditional extraction kits, DNAreleasy Advance does not purify the DNA; instead, it stabilises the lysate so that it can be added directly to a PCR mix. The formulation is compatible with a wide variety of sample types, including mammalian cells, saliva, hair roots, animal tissues, bacteria, yeast, and plants.
Key Benefits:
- Rapid Protocol: 15-minute turnaround. The standard lysis profile requires only 15 minutes in a thermal cycler (5 minutes at 65°C, 5 minutes at 96°C, 5 minutes at 20°C).
- Simplified Workflow: No purification required. The lysate is used directly in subsequent PCR reactions, eliminating the need for spin columns, ethanol precipitation, or buffer exchanges.
- Broad Compatibility: Versatile sample types. Successfully releases DNA from diverse sources including Drosophila, mammalian tissues (e.g., mouse tails), yeast, and bacteria.
- High Efficiency: Direct PCR integration. The resulting lysate can comprise up to 10% of the total volume of most standard PCR mixes without inhibiting the reaction.
- Flexible Storage: Convenient stability. Reagents can be stored at 4°C for up to one month for frequent use, or -20°C for long-term storage.
Supporting Data
The DNAreleasy Advance simplifies the workflow significantly compared to standard isolation methods.
Genomic DNA from scallops
Genomic DNA scallops was isolated by DNAreleasy Advance and a part of the supernatant was directly added to the PCR reaction. The agarose gel shows the high yield of amplified DNA fragment.
The same amplified DNA was used for subsequent sequencing experiments.
Data kindly provided by Dr. Schubbert, Eurofins Medigenomix GmbH, Ebersberg, Germany
Quantitative PCR of plant tissues
Total DNA was isolated by DNAreleasy Advance from various plant species. Thereafter the melting curve was determined by RT PCR by using a Light Cycler instrument (Roche):
Positive control (PTC), maize meal, wheat flour, sugar beet, white cabbage; negative control (NTC)
Human and animal genomic DNA
Genomic DNA was isolated by using DNAreleasy Advance from different materials and organism and analyzed by Roche Light Cycler:
(1) positive control human DNA, (2) saliva, (3) hair root, (4) pig liver, (5) drosophila melangogaster, (6) horse meat
Specifications
- Product Form: Liquid lysis reagent
- Pack Sizes: 0.3 ml (10 reactions) or 1.5 ml (50 reactions)
- Protocol Time: ~15 minutes (standard); ~40 minutes (difficult tissues)
- Lysis Volume: 30 µl per sample
- Storage Conditions: -20°C for long-term; 4°C for up to 1 month
- PCR Compatibility: Lysate can make up to 10% of subsequent PCR mix
- Usage: Research Use Only