Biofilm DNA Isolation Kit: Expert Solution for Complex Microbial Matrices
The Biofilm DNA Isolation Kit (62300) is a specialised system engineered for the rapid and efficient preparation of genomic DNA from diverse biofilm types and biofilm-forming bacteria. Biofilms present significant challenges for standard extraction methods due to their dense extracellular polymeric substances (EPS) and the presence of potent PCR inhibitors like humic acids. This kit provides a robust solution, ensuring the delivery of high-titer, high-purity DNA that is ready for demanding molecular applications including qPCR, NGS, and Southern blotting.
Purification is powered by advanced spin column chromatography utilising a proprietary resin matrix. The process begins with a synergistic combination of heat treatment and chemical detergents, followed by mechanical homogenisation in provided Bead Tubes. This multi-modal lysis strategy effectively disrupts the complex biofilm architecture and bacterial cell walls. Once the DNA is bound to the column, a series of optimised wash steps removes proteins and contaminants, while the specific chemistry of the resin ensures the elimination of humic acids and other inhibitory substances before final elution.
Key Benefits:
- Rapid Workflow: Complete isolation in 45 minutes. Process up to 10 samples simultaneously in under an hour, significantly reducing hands-on time compared to traditional methods.
- Superior Inhibitor Removal: Pure DNA for sensitive assays. Effectively eliminates humic acids, polyphenols, and polysaccharides that typically interfere with PCR and sequencing.
- High-Quality Yields: Optimised for difficult samples. Recovers 2–4 μg of high-molecular-weight DNA from as little as 200 mg of biofilm wet weight.
- Universal Compatibility: Broad microbial coverage. Validated for use with various species, including Komagataeibacter and other complex biofilm-forming organisms.
- Safe and Non-Toxic: No hazardous extractions. Eliminates the need for phenol, chloroform, or caesium chloride, ensuring a safer laboratory environment.
Technical Insights
Figure 1: High Purity and Integrity of Biofilm DNA
Analysis of genomic DNA isolated from various biofilm samples using the Geneflow Biofilm DNA Isolation Kit. The results demonstrate consistent high-molecular-weight bands with no detectable degradation, confirming the kit’s ability to maintain DNA integrity throughout the lysis and purification stages.
Figure 1. High DNA quality. DNA was isolated from a number of bioflim using Norgen’s Biofilm DNA Isolation Kit. All DNA elutions isolated using Norgen’s Biofilm DNA Isolation Kit showed an excellent DNA quality indicating the consistent and robust performance of the Biofilm DNA Isolation Kit.
Figure 2. High quality and yield of DNA from biofilm formed by two different species, Komagataeibacter xylinus (1-4), Komagataeibacter hansenii (5-8). Total DNA was isolated from 200 mg of biofilm using Norgen’s Biofilm DNA Isolation Kit. For evaluation, 10 µL of each 100 µL DNA elution was run on a 1.2 % agarose gel. Note the high yield and quality of the DNA in all lanes. Lane M: Norgen’s HighRanger 1 kb DNA Ladder (cat. 11900).
Specifications
- Technology: Proprietary Spin Column Chromatography
- Sample Type: Biofilms, Microbial Mats, Biofilm-forming Bacteria
- Sample Input: 50 mg to 200 mg (Wet Weight)
- Binding Capacity: 50 μg per column
- Processing Time: 45 minutes for 10 samples
- Typical Yield: 2–4 μg (from 200 mg sample)
- Storage: Room Temperature (15–25°C)
- Shelf Life: 12 months from date of shipment