UreaGel 8: High-Resolution 8% Ready-to-Cast Denaturing PAGE Solution
UreaGel 8 (EC-838) is a premium, pre-mixed working solution formulated for the rapid and reproducible preparation of 8% denaturing polyacrylamide gels. Engineered specifically for high-resolution DNA sequencing and fragment analysis, this solution provides the ideal matrix density for separating medium-to-small nucleic acid fragments. By integrating the optimal 19:1 acrylamide to bis-acrylamide ratio, 7.5M urea, and 1X TBE buffer into a single liquid, UreaGel 8 eliminates the variability and hazards associated with manual reagent mixing.
The mechanism of UreaGel 8 relies on a stabilized 7.5M urea environment that ensures complete denaturation of nucleic acids throughout the electrophoresis run. This high concentration of urea prevents the formation of secondary structures in DNA and RNA, which is critical for achieving sharp, distinct bands and accurate migration data. As a deionised and 0.2-micron filtered solution, it provides a superior signal-to-noise ratio, ensuring that even low-abundance bands are clearly visible against a crystal-clear background. This ready-to-use format modernises the laboratory by removing the need for deionising urea stocks or handling neurotoxic acrylamide powders.
Key Benefits:
- Optimised 8% Matrix: Ideal for sequencing. Specifically designed for the resolution required in DNA sequencing and oligonucleotide fragment analysis.
- Stabilised Denaturation: Constant 7.5M Urea. Ensures complete suppression of secondary structures for reliable, single-strand migration patterns.
- “Plug-and-Play” Convenience: No mixing required. Pre-equilibrated with 1X TBE and urea, allowing researchers to cast gels immediately upon adding polymerisation catalysts.
- Nuclease-Free Assurance: Certified purity. Strictly tested for RNase and DNase contamination to protect the integrity of sensitive genetic samples during long-run analysis.
- Enhanced Lab Safety: Liquid-based system. Eliminates the risk of inhalation exposure to hazardous acrylamide dust and streamlines waste management protocols.
Technical Insights
Simplified Casting and Polymerisation
Using UreaGel 8 significantly reduces the complexity of gel casting. Because the solution contains all necessary buffer and denaturant components at their final working strengths, the only required additions are TEMED and Ammonium Persulfate (APS) to initiate polymerisation. This “single-bottle” approach ensures that every gel cast in the lab has identical ionic strength and denaturing capacity, virtually eliminating the “smiling” effects caused by hand-mixed reagent inconsistencies.
Specifications
- Catalogue Number: EC-838
- Final Concentration: 8% Acrylamide/Bis-acrylamide
- Cross-linker Ratio: 19:1 (Acrylamide:Bis)
- Denaturant: 7.5M Urea
- Buffering Agent: 1X TBE (Tris-Borate-EDTA)
- Physical Format: Deionised, 0.2 micron filtered liquid
- Certification: Certified Nuclease-Free (RNase/DNase)
- Storage: Room Temperature (18-25°C), protected from light
- Shelf Life: 12 months from date of manufacture