TransIT®-Keratinocyte Transfection Reagent: Specialised DNA Delivery for Skin Cells
The TransIT®-Keratinocyte Transfection Reagent is a specialised formulation specifically optimised to provide exceptional transfection efficiency of plasmid DNA in primary and immortalised keratinocytes. Keratinocytes are often challenging to transfect due to their sensitivity and specific growth requirements. This reagent addresses these challenges by delivering high-performance results while maintaining the physiological integrity of the cells, making it an essential tool for dermatological and epithelial research.
Designed with the researcher in mind, TransIT-Keratinocyte combines high transfection efficiency with low toxicity and reproducibility. The protocol is streamlined for simplicity; transfection does not require medium changes and can be carried out directly in serum-containing medium. This feature minimises handling and stress on the cells, ensuring optimal viability. The reagent is suitable for both transient and stable transfection applications, offering versatility for various experimental designs.
Key Benefits:
- Cell-Type Specificity: Optimised for Keratinocytes. Specifically formulated to maximise DNA delivery in both primary and immortalised keratinocyte lineages.
- Superior Viability: Low Toxicity. The gentle formulation ensures high transfection efficiency without compromising cell health or morphology.
- Streamlined Workflow: Serum Compatible. Performed in complete growth medium without the need for a post-transfection medium change, saving time and reducing contamination risk.
- Versatile Application: Transient & Stable. Proven effective for short-term expression studies as well as the generation of stable cell lines.
- Consistent Performance: Reproducible Results. Provides all the attributes of the trusted TransIT series, ensuring consistent data across experiments.
Technical Insights
Protocol Optimisation
To achieve the best results, the reaction conditions can be optimised for specific keratinocyte subtypes. While the recommended starting condition is 3 µl of TransIT-Keratinocyte Reagent per 1 µg of DNA, researchers can titrate the reagent volume from 2–8 µl per 1 µg of DNA to determine the optimal ratio. For maximum efficiency, it is recommended to divide cells 18–24 hours before transfection to ensure they are actively dividing and reach approximately ≥80% confluence at the time of transfection.
Specifications
- Product Type: Transfection Reagent
- Storage Conditions: Store at 4°C. Warm to room temperature and vortex gently before each use.
- Shelf Life: 1 year from the date of purchase when properly stored and handled.
- Reagent Compatibility: Compatible with serum-containing medium (complete growth medium).
- Antibiotic Compatibility: Transfection complexes can be added to cells grown in medium containing low levels of antibiotics (0.1-1X).
- Ordering Information:
- E7-0084: 1 x 0.4 ml (MIR 2804)
- E7-0082: 1 x 1.0 ml (MIR 2800)
- E7-0086: 5 x 1.0 ml (MIR 2805)
- E7-0088: 10 x 1.0 ml (MIR 2806)