TransIT®-293 Transfection Reagent: Superior Efficiency for HEK 293 Lineages
TransIT®-293 Transfection Reagent is a specialised formulation specifically optimised to provide exceptional transfection efficiency of plasmid DNA in HEK 293 cells and associated lineages. HEK 293 cells are a workhorse in biopharmaceutical research, widely used for protein expression and viral vector production. This reagent is engineered to meet the high demands of these applications, delivering consistent, high-level expression required for scale-up and manufacturing.
Combining the attributes of the trusted TransIT series, TransIT-293 offers low toxicity and complete serum compatibility. Transfections can be performed directly in complete growth medium without the need for a post-transfection medium change, significantly reducing hands-on time. Whether you are producing recombinant viruses or analysing promoter activity, TransIT-293 provides the robust performance needed for reproducible success in HEK 293 cultures.
Key Benefits:
- Cell-Line Specific: Optimised for 293. Specifically tuned to maximise DNA delivery and expression in HEK 293 cells and derivatives (e.g., 293T, 293F).
- High Performance: Superior Efficiency. Consistently achieves high transfection rates (75-85%), ensuring reliable data for critical experiments.
- Production Ready: High Yields. Ideal for applications requiring high protein titers or efficient viral packaging.
- Streamlined Workflow: Serum Compatible. Eliminates the need for media changes; compatible with serum-containing environments.
- Cell Health: Low Toxicity. Maintains high cell viability, which is essential for sustained protein production and viral harvest.
Technical Insights
Protocol Optimisation
For optimal results in HEK 293 subtypes, the TransIT-293 protocol recommends a starting ratio of 3 µl of reagent per 1 µg of DNA. Researchers can optimise this ratio within a range of 2–6 µl per 1 µg DNA. It is recommended that cells be approximately ≥80% confluent at the time of transfection to ensure active division and maximum uptake.
Specifications
- Product Type: Transfection Reagent (HEK 293 Specific)
- Storage Conditions: Store tightly capped at 4°C. Warm to room temperature and vortex gently before use.
- Shelf Life: 1 year from the date of purchase when stored properly.
- Reagent Compatibility: Compatible with serum-containing medium.
- Ordering Information:
- E7-0028: 1 x 0.4 ml (MIR 2704)
- E7-0026: 1 x 1.0 ml (MIR 2700)
- E7-0030: 5 x 1.0 ml (MIR 2705)
- E7-0032: 10 x 1.0 ml (MIR 2706)