SSC Buffer (20 X): Reliable Saline-Sodium Citrate for Blotting
National Diagnostics SSC Buffer (20 X) is a vital reagent for the transfer and hybridization of nucleic acids in Southern and Northern blotting procedures. Achieving a successful blot requires a precise ionic environment to facilitate the movement of DNA or RNA from a gel to a membrane. This 20 X concentrate provides the high-purity Saline-Sodium Citrate needed to ensure efficient binding and specific probe hybridization.
The buffer functions by providing a high concentration of sodium ions (NaCl), which neutralises the negative charge of the nucleic acid backbone, thereby facilitating its adsorption onto nitrocellulose or nylon membranes. The sodium citrate component acts as a buffer to maintain a pH of 7.0, preventing the depurination of DNA that can occur in more acidic environments. By using a pre-mixed 20 X concentrate, laboratories ensure the consistent ionic strength necessary for sensitive hybridization assays.
Key Benefits:
- Optimal Transfer Efficiency: Consistent ionic strength. Maximises the transfer of DNA and RNA from agarose gels to membranes.
- Precise pH Control: Neutralises and stabilises. Maintains a stable pH 7.0 to protect the integrity of the nucleic acid during long transfer steps.
- Versatile Application: Multi-purpose utility. Ideal for use in transfer, blocking, and hybridization wash steps.
- Strict Quality Control: Ultra-pure formulation. Filtered and certified to be free from contaminants that could cause background interference on blots.
Specifications
- Concentration: 20 X Liquid Concentrate
- Composition: 3M Sodium Chloride, 0.3M Sodium Citrate (pH 7.0 at 1X)
- Storage: Store at room temperature (15-30°C)
- Shelf Life: 24 months
- Application: Southern Blotting, Northern Blotting, In-situ Hybridization