SDS-PAGE Tank Buffer (10X): High-Resolution Protein Electrophoresis
The Geneflow SDS-PAGE Tank Buffer (10X) is a premium Tris-Glycine-SDS formulation designed to standardise and streamline protein electrophoresis workflows. Inconsistent buffer preparation is a common source of gel artefacts and poor band resolution. This pre-mixed, highly concentrated solution addresses these challenges by providing a precisely balanced ionic environment, eliminating the weighing errors and batch-to-batch variability associated with preparing Laemmli buffers from powder.
The buffer operates on the classic discontinuous buffer system principles. It provides the essential chloride and glycine ions required to stack and resolve proteins effectively. The inclusion of high-quality Sodium Dodecyl Sulphate (SDS) ensures uniform negative charge distribution across protein samples, maintaining their denatured state and facilitating migration based solely on molecular weight. This optimised formulation guarantees crisp, straight bands and reproducible relative mobility (Rf) values.
Key Benefits:
- Enhanced Reproducibility: Consistent Ionic Strength. Manufactured under strict quality control to ensure batch-to-batch consistency, minimising experimental variability.
- Superior Resolution: Sharper Bands. High-purity reagents reduce background smearing and improve the clarity of protein bands for publication-quality imaging.
- Workflow Efficiency: Ready-to-Dilute Format. The 10X concentrate saves preparation time and reduces the risk of inhalation hazards associated with handling loose SDS powder.
- Versatile Application: Broad Compatibility. Ideal for standard SDS-PAGE, Western Blotting applications, and use with a wide range of pre-cast or hand-cast gels.
Technical Insights
Buffer Chemistry and Migration
Upon dilution to a 1X working concentration, this buffer typically yields a pH of approximately 8.3. This pH is critical as it regulates the ionisation state of glycine. In the stacking gel, glycine exists as a zwitterion (net neutral charge), moving slowly and creating a voltage steep that compacts proteins into a tight disc. Upon entering the resolving gel (higher pH), glycine becomes negatively charged and moves ahead of the proteins, allowing separation by size in the sieving matrix.
Specifications
- Concentration: 10X (Dilute 1:9 with deionised water).
- Composition: Tris-Base, Glycine, Sodium Dodecyl Sulphate (SDS).
- pH (1X): 8.3 ± 0.2 @ 25°C.
- Storage: Store at Room Temperature (15°C to 25°C). Do not refrigerate to prevent SDS precipitation.
- Shelf Life: 12 months from date of manufacture.
- Appearance: Clear, colourless liquid.