Low Abundance DNA Quantification Kit: High-Sensitivity Detection for Demanding Samples
When working with challenging liquid biopsy samples such as urine or plasma, researchers frequently encounter DNA yields that fall well below the detection limits of traditional spectrophotometry. The Low Abundance DNA Quantification Kit provides an exceptionally sensitive PCR-based method, allowing for the precise measurement of DNA across a broad spectrum of concentrations, including the difficult-to-detect lower ng/µL, pg/µL, and sub-pg/µL ranges.
This kit relies on a highly optimised, specially designed primer mix that functions in conjunction with the provided 2X PCR Master Mix to amplify human DNA. It is fully compatible with any Real-Time PCR system utilising standard fluorescent nucleic acid binding dyes, such as SYBR Green. By constructing a standard curve using the supplied quantified DNA standards, users can reliably determine the exact concentration of their unknown samples, ensuring downstream experimental success.
Key Benefits:
- Exceptional Sensitivity: Detect sub-picogram quantities. Accurately quantify purified DNA from low-abundance samples down to 500 fg, significantly outperforming traditional absorbance-based methods.
- Broad Dynamic Range: Versatile sample processing. Measure DNA concentrations across a wide spectrum, spanning from the lower nanogram scale down to the sub-picogram per microlitre range.
- Universal Compatibility: Integrates into existing workflows. Fully compatible with any Real-Time PCR (qPCR) instrument when used with standard fluorescent dyes like SYBR Green or FAM.
- Absolute Quantification: Reliable and reproducible results. Accurately calculate unknown DNA concentrations using a robust standard curve generated from the pre-quantified, five-point DNA standard included in the kit.
Technical Insights
Traditional spectrophotometers and even some fluorescent dye-based systems struggle to quantify DNA below 1-10 ng/µL. The PCR-based methodology of the Low Abundance DNA Quantification Kit entirely bypasses these optical limitations, extending the reliable detection threshold directly into the femtogram scale. This level of precision is particularly vital when preparing samples for Next-Generation Sequencing (NGS) or sensitive genotyping applications, where precise template input is critical to generating robust data without amplification bias.
▼ View Technical Data Figures
Figure 1. Sensitivity of DNA Quantification of the Low Abundance DNA Quantification Kit Compared to Other Methods
A diagram representing the dynamic range of different DNA quantification methods is presented here. The Low Abundance DNA Quantification Kit can quantify purified DNA from low abundance samples in the pg and sub-pg range.
Figure 2. Sensitivity of DNA Quantification in the Picogram Range Using the Low Abundance DNA Quantification Kit
A representative qPCR Baseline Graph showing the amplification of a DNA standard dilution series. The Low Abundance DNA Quantification Kit can quantify purified DNA from low abundance samples such as liquid biopsies (Plasma or Urine). As little as 500 fg of DNA can be quantified.
Specifications
- Format: PCR-based detection
- Pack Size: 48 Reactions
- Sample Compatibility: Purified DNA from various biological sources (highly recommended for liquid biopsies, plasma, serum, and urine)
- Detection Limit: Down to the sub-pg/µL range (approximately 500 fg)
- Kit Contents: 2X PCR Master Mix (1 mL), DNA Quantification Primer Set Mix (200 µL), Quantified DNA Standard (5 x 100 µL), Nuclease-Free Water (1.25 mL)
- Storage Conditions: Store at -20°C or lower upon receipt. Avoid multiple freeze-thaw cycles by preparing smaller working aliquots.