Ingenio® Electroporation Kits: Universal High-Efficiency Transfection
The Ingenio® Electroporation Kits and Solution provide a high-performance, universal alternative for the electroporation of DNA and siRNA into mammalian cells. Designed specifically for researchers working with hard-to-transfect cell types—such as primary cells, stem cells, and suspension lines—this solution overcomes the traditional challenges of low transfection efficiency and high cellular toxicity. By providing a stable, optimised environment for permeabilisation, Ingenio® ensures that valuable genetic material is delivered effectively without compromising cell viability.
One of the defining advantages of the Ingenio® product range is its broad compatibility. The solution is formulated to work seamlessly with multiple conventional electroporation instruments, including the Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser®, and Harvard-BTX® systems. Whether your laboratory utilises exponential decay or square wave pulses, Ingenio® allows you to use a single, cost-effective reagent across different platforms and cell types, simplifying your workflow while maintaining high-quality gene expression results.
Key Benefits:
- High Efficiency Delivery: Optimised for difficult targets. Delivers DNA and siRNA with exceptional efficiency into hard-to-transfect cells, including Jurkat E6-1, primary human keratinocytes, and varying stem cell lines.
- Universal Compatibility: Open system flexibility. Fully compatible with major electroporators such as the Amaxa Nucleofector (I, II/2B), Bio-Rad Gene Pulser, and BTX instruments, allowing you to transition away from expensive, instrument-specific kits.
- Low Cellular Toxicity: Maintains cell health. The formulation is designed to minimise cell death associated with electroporation, ensuring a higher yield of viable, expressing cells post-transfection.
- Cost-Effective Solution: Reduce operational costs. Available as a standalone solution or in complete kits with cuvettes and droppers, offering significant savings compared to proprietary system-specific consumables.
- Simplified Protocol: Streamlined workflow. A single, universal solution replaces the need for multiple cell-type-specific buffers, reducing inventory complexity and preparation time.
Technical Insights
The Ingenio® Electroporation Solution has been rigorously tested to ensure robust performance across a diverse range of cell lineages. The formulation supports both exponential decay and square wave pulse conditions, providing flexibility for protocol optimisation.
Optimised Performance Across Platforms:
Internal validation demonstrates that Ingenio® serves as a direct functional replacement for standard Nucleofector® solutions. When using the Amaxa® Nucleofector®, researchers can utilise standard 0.2 cm cuvettes and validated program settings (e.g., Program X-001 for Jurkat E6-1 or T-020 for Primary MEFs) to achieve comparable or superior transfection efficiencies.
Specifications
- Storage Conditions: Store Ingenio® Electroporation Solution at 4°C. Cuvettes and droppers may be stored at room temperature.
- Shelf Life: Guaranteed for 1 year from the date of purchase when stored and handled properly.
- Cuvette Compatibility: Kits are available with standard 0.2 cm (100 µl volume) or 0.4 cm (250 µl volume) gap cuvettes.
- Instrument Compatibility: Validated for use with Mirus Bio Ingenio® EZporator®, Lonza Amaxa® Nucleofector®, Bio-Rad® Gene Pulser®, and Harvard-BTX® electroporators.
- Pulse Compatibility: Compatible with both exponential decay and square wave pulse forms.
- Kit Formats: Available as standalone solution or kits containing 25, 50, or 100 reactions (includes solution, cuvettes, and transfer droppers).