Food DNA Isolation Kit – 54500: Rapid GMO & Pathogen Detection
The Food DNA Isolation Kit (54500) provides a rapid, robust spin-column method for the isolation and purification of total DNA from a wide range of food samples originating from both animals and plants. Designed for laboratories conducting essential food safety and quality analyses, this kit is highly effective for the identification of GMO-DNA or specific animal components in food and feed. It easily accommodates a broad spectrum of complex starting materials, from raw and processed meats to liquids, sauces, and dairy products including milk, cheese, and yogurt.
The underlying technology utilises a proprietary resin that binds nucleic acids based on ionic concentrations, cleanly separating high-molecular-weight DNA from proteins and other cellular components without the use of hazardous chemicals like phenol or chloroform. Following a highly effective lysis step utilizing the included Proteinase K, the streamlined protocol allows researchers to isolate pristine, inhibitor-free DNA in under 45 minutes. Furthermore, this kit provides a highly convenient and universal method for isolating DNA from contaminating food-related pathogens (including Gram-positive and Gram-negative bacteria, yeast, and fungi) ensuring the purified DNA is perfectly suited for downstream applications such as PCR, sequencing, and genotyping.
Key Benefits:
- Rapid & Convenient: Less than 15 minutes hands-on time. The efficient spin-column format significantly reduces processing time, allowing for rapid sample turnover in high-throughput environments.
- Uncompromised Safety: Phenol and chloroform free. Avoid the health hazards and disposal costs associated with toxic organic solvents, ensuring a safer laboratory environment.
- Broad Sample Compatibility: Isolate from complex matrices. Process raw, boiled, fluid, or processed food products efficiently, making it an ideal universal solution for GMO and authenticity testing.
- Pathogen Detection Ready: Universal microorganism isolation. Effectively isolates DNA from enriched or native foodborne pathogens (e.g., E. coli O157:H7, Listeria, Salmonella) for rapid diagnostic PCR.
- High-Purity Yields: Inhibitor-free DNA. Delivers exceptional DNA quality (A260/280 ratios of 1.9–2.1) ensuring reliable performance in sensitive downstream enzymatic reactions.
Technical Insights
Optimised Sample Input
To ensure maximum yield and prevent column clogging, the protocol is highly optimised for specific input amounts. For solid food materials, an input of 200 mg is recommended, while liquid samples (such as milk or concentrated juices) should be limited to 1.0 mL to 1.5 mL. Adhering to these specifications guarantees that the lysis and binding buffers maintain the correct ionic balance for optimal DNA capture on the spin column.
Specifications
- Product Type: Spin-Column DNA Isolation Kit
- Maximum Column Binding Capacity: 50 µg
- Maximum Column Loading Volume: 650 µL
- Maximum Starting Material (Solid): 200 mg
- Maximum Starting Material (Liquid): 1.0 mL to 1.5 mL
- Processing Time: 45 minutes (for 10 purifications)
- Format: 50 Preparations
- Storage: All components (including the specially buffered Proteinase K) are stable at room temperature for up to 1 year.