Cytoplasmic & Nuclear RNA Purification Kit

Cytoplasmic & Nuclear RNA Purification Kit: Precision Fractionation for Enhanced Transcriptomics

The Cytoplasmic & Nuclear RNA Purification Kit provides a streamlined solution for researchers requiring distinct isolation of RNA from cellular compartments. Traditionally, separating cytoplasmic and nuclear fractions is a laborious process prone to cross-contamination; however, this kit simplifies the workflow to ensure high-purity RNA ready for immediate downstream applications. By isolating DNA-free cytoplasmic RNA and high-quality nuclear RNA, scientists can more accurately study RNA transport, processing, and the localisation of specific transcripts within cultured animal cells and small tissue samples.

Utilising advanced spin-column chromatography, the kit operates without the need for hazardous phenol or chloroform extractions. The technology is engineered to capture the full spectrum of RNA sizes, including large mRNA and ribosomal RNA down to microRNA (miRNA) and short interfering RNA (siRNA). This comprehensive recovery ensures that the resulting fractions represent the true biological state of the sample, providing an essential tool for transcriptomic analysis, gene regulation studies, and RNA-seq workflows.

Key Benefits:

• Rapid Workflow: Process 10 samples in 45 minutes. The efficient protocol minimises RNA degradation and saves valuable laboratory time compared to traditional fractionation methods.
• Superior Purity: DNA-free cytoplasmic RNA. The specialised separation process ensures that the cytoplasmic fraction is ready for RT-PCR and qRT-PCR without the requirement for additional DNase treatment.
• No Phenol Required: Safe and optimised protocol. Eliminates the health risks and disposal issues associated with organic solvents while maintaining high yields and RNA integrity.
• Small RNA Recovery: Capture the complete RNA profile. Unlike many standard kits, this resin-based technology includes small RNA species (<200 nt) such as miRNA and siRNA in both cellular fractions.
• Versatile Application: Optimised for various inputs. Suitable for a wide range of cultured animal cells and small tissue biopsies, ensuring consistent results across different experimental models.

Technical Insights

The separation of nuclear and cytoplasmic RNA is achieved through a gentle lysis buffer that selectively disrupts the plasma membrane while leaving the nuclear envelope intact. After centrifugation, the cytoplasmic fraction is collected as the supernatant, while the nuclei are pelletised for subsequent lysis. Both fractions are then purified using Norgen’s proprietary resin technology, which binds RNA based on size and charge without the bias often seen in silica-based methods. This ensures that even the most delicate regulatory RNAs are preserved and concentrated for high-sensitivity detection.

Specifications

• Column Binding Capacity: 50 μg
• Maximum Column Loading Volume: 650 μL
• Size of RNA Purified: All sizes, including small RNA (<200 nt)
• Time to Complete 10 Purifications: 45 minutes
• Application: RT-PCR, qRT-PCR, RNA-Seq, Northern Blotting
• Storage Conditions: Room Temperature ($15-25^\circ C$)
• Shelf Life: 12 months from date of shipment