AccuGel 19:1: High-Resolution 40% Acrylamide/Bis-Acrylamide Solution
AccuGel 19:1 (EC-850) is a premium, ready-to-use 40% (w/v) aqueous solution of acrylamide and methylene bis-acrylamide, precisely formulated at a 19:1 ratio. Traditional polyacrylamide gel preparation often involves the hazardous handling of neurotoxic powdered reagents, which presents a significant risk of inhalation from airborne particulates. AccuGel eliminates these safety concerns and the potential for weighing errors by providing a pre-mixed, ultra-pure liquid format that ensures consistent, reproducible gel matrices every time.
The superior performance of AccuGel is driven by its advanced purification and stabilisation process. It is specifically treated to have zero acrylic acid content, removing the fixed charges that typically cause band streaking and electroendosmosis. Furthermore, oxidation products such as aldehydes and metal ion contaminants are removed via a selective adsorption process. This results in a crystal-clear solution that provides a neutral, stable matrix for high-resolution separation of nucleic acids and proteins, maintaining its integrity throughout an extended shelf life.
Key Benefits:
- Unmatched Lab Safety: Zero Dust Hazard. By replacing toxic powdered acrylamide with a liquid solution, you eliminate the risk of inhaling neurotoxic dust during preparation.
- Superior Band Clarity: Zero Acrylic Acid. The removal of ionic impurities prevents band distortion and “smiling” effects, ensuring sharp, publication-quality results.
- Optimised Consistency: Precise 19:1 Ratio. Pre-mixed under strict quality controls to guarantee uniform cross-linking and reproducible pore sizes for every gel run.
- Long-Term Stability: Stabilised Formulation. Proprietary stabilisation prevents spontaneous polymerisation and hydrolysis, offering a reliable shelf life of up to 24 months.
- Durable Packaging: Shatter-Proof Protection. Supplied in PVC-coated bottles to prevent accidental breakage and chemical exposure in the laboratory.
Technical Insights
Precision Cross-Linking for Nucleic Acid Analysis
The 19:1 ratio in AccuGel 19:1 provides a higher degree of cross-linking compared to standard 29:1 solutions. This makes it particularly effective for applications requiring high-resolution separation of small DNA and RNA fragments, such as sequencing or mutation detection. The ultra-pure nature of the solution ensures that the resulting polyacrylamide matrix is homogeneous and free from particulates that could interfere with sensitive imaging or downstream blotting techniques.
Specifications
- Composition: 40% (w/v) Acrylamide / Bis-Acrylamide
- Cross-linker Ratio: 19:1 (Acrylamide:Bis)
- Purity: Zero acrylic acid and aldehyde-free
- Filtration: 0.2 µm filtered for high clarity
- Storage: Store at 4°C (Do not freeze)
- Shelf Life: 24 months from date of manufacture
- Packaging: PVC-coated shatter-proof bottles