P4-0422
2X One-Step RT-PCR Master Mix – 28113

Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates.
P4-0422 Image
Catalogue NumberDescriptionPack SizePrice
P4-04222X One-Step RT-PCR Master Mix – 28113100 Reactions£241.30
  • Convenient ready-to-use solution
  • High sensitivity and yield
  • Robust amplification
  • 20 µL volume reactions
  • This product is a recommended add-on to be used with Norgen's COVID-19 Primer & Probe Mixes and COVID Positive Control 

2X One-Step RT-PCR Master Mix

Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required  for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA temples with high yields of PCR products. Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Setp RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV.
 
 

Specification

Specification
Reagents supplied – Cat # 28113/P4-0422 2X One-Step RT-PCR Master Mix (1 Vials, 100 Reactions) - Sufficient reagent for 100 x 20 µL reactions Storage Conditions and Product Stability 2X One-Step RT-PCR Master Mix should be stored at -20°C. For everyday use an aliquot can be stored at 4°C for up to 3 months. Repeated freeze-thaw cycles are not recommended. When stored at the proper temperature this reagent is stable for at least 1 year.

Documentation

Literature
P4-0422 Literature 28113
Protocol
P4-0422 Protocol 28113
Catalogue NumberDescriptionPack SizePrice
P4-0058Total RNA Purification Kit – 1720050 Preps£287.00
P4-04242019-nCoV TaqMan RT-PCR Kit (CDC Version) – TM6710050 Reactions£298.00
P4-04182019-nCoV Primer & Probe Mixes – TM6710150 Reactions£110.35
P4-04222X One-Step RT-PCR Master Mix – 28113100 Reactions£241.30