Urine Cell-Free Circulating & Viral Nucleic Acid Purification Maxi Kit: Comprehensive Biomarker Recovery from Large-Volume Urine Samples
The Urine Cell-Free Circulating & Viral Nucleic Acid Purification Maxi Kit provides a rapid and highly efficient solution for isolating high-quality, inhibitor-free nucleic acids from 10 mL to 30 mL urine samples. Designed for researchers focusing on non-invasive diagnostics and liquid biopsies, this kit facilitates the purification of circulating cell-free DNA (cfc-DNA), exosomal RNA, and viral DNA/RNA from fresh, frozen, or preserved specimens. By addressing the challenge of low-concentration biomarkers in urine, this Maxi kit ensures high recovery rates and exceptional purity, even from large input volumes.
Purification is based on an advanced spin column chromatography method using a proprietary resin separation matrix. This technology eliminates the need for hazardous phenol/chloroform extractions or time-consuming protease treatments, significantly streamlining the laboratory workflow. The resin selectively binds all sizes of nucleic acids, including microRNA and short circulating fragments, which are then eluted into flexible volumes ranging from 50 µL to 100 µL. This results in highly concentrated, purified nucleic acids of the highest integrity, ready for immediate use in sensitive downstream applications.
Key Benefits:
- Broad Spectrum Isolation: Captures all sizes of DNA and RNA. Recovers everything from microRNA and small circulating fragments to high-molecular-weight DNA and exosomal RNA.
- High Concentration Efficiency: Maximises biomarker recovery from large volumes. Process input volumes of 10 mL to 30 mL and elute into as little as 50 µL to ensure highly concentrated samples for analysis.
- Chemical-Free Processing: No phenol or chloroform required. Eliminates the use of hazardous organic solvents, improving laboratory safety and simplifying the purification protocol.
- Superior Purity: Delivers inhibitor-free nucleic acids. Effectively removes common urinary inhibitors to ensure reliable performance in sensitive assays such as NGS, qPCR, and methylation studies.
Specifications
- Catalogue Number: P4-0381
- Input Volume: 10 mL to 30 mL urine
- Sample State: Fresh, frozen, or preserved urine
- Elution Volume: 50 µL to 100 µL
- Technology: Spin column chromatography (Proprietary resin)
- Downstream Compatibility: PCR, qPCR, NGS, Southern/Northern Blots, and methylation-sensitive assays