Urine Cell-Free Circulating & Viral Nucleic Acid Purification Mini Kit: High-Sensitivity Recovery for Small-Volume Liquid Biopsies
The Urine Cell-Free Circulating & Viral Nucleic Acid Purification Mini Kit provides a fast, reliable, and convenient method for the isolation of high-quality, inhibitor-free nucleic acids from small urine volumes ranging from 250 µL to 2 mL. As the demand for non-invasive diagnostic tools grows, this kit offers a streamlined solution for researchers needing to extract circulating cell-free DNA (cfc-DNA), circulating RNA (including exosomal and microRNA), and viral DNA/RNA. By facilitating the concentration of these low-abundance biomarkers from fresh, frozen, or preserved urine samples, it enables high-resolution molecular analysis even from limited sample sizes.
The purification mechanism is based on advanced spin column chromatography utilising a proprietary resin separation matrix. Unlike traditional methods, this technology does not require hazardous phenol/chloroform extractions or time-consuming protease treatments, significantly reducing hands-on time and improving laboratory safety. The resin is specifically engineered to capture all sizes of nucleic acid fragments with high affinity. Following a rapid wash protocol to remove urinary inhibitors, the target analytes are eluted in flexible volumes of 50 µL to 100 µL, resulting in highly concentrated samples ready for the most sensitive downstream applications.
Key Benefits:
- Versatile Multi-Analyte Extraction: Simultaneous isolation of DNA and RNA. Efficiently recovers cfc-DNA, cfc-RNA, exosomal RNA, and viral nucleic acids in a single, unified protocol.
- Small Volume Optimisation: Maximises yield from limited samples. Specifically designed for input volumes between 250 µL and 2 mL, making it ideal for clinical pilot studies and precious biobanked specimens.
- Clean and Safe Protocol: Phenol-free and protease-free workflow. Eliminates the need for toxic organic solvents and lengthy digestion steps, ensuring a faster path from sample to result.
- Assay-Ready Purity: Superior removal of urinary inhibitors. Delivers nucleic acids of the highest integrity, ensuring reliable performance in NGS, qPCR, and methylation-sensitive assays.
Specifications
- Catalogue Number: P4-0379
- Input Volume: 250 µL to 2 mL urine
- Sample Type: Fresh, frozen, or preserved urine
- Elution Volume: 50 µL to 100 µL
- Technology: Spin column chromatography (Proprietary resin)
- Downstream Compatibility: PCR, qPCR, NGS, Southern/Northern Blotting, and methylation studies