HR Agarose, PCR Grade: Precision Analysis for Small Fragments
Geneflow HR Agarose, PCR Grade (CleverGEL), is a high-purity agarose specifically engineered for the ultra-high resolution of small nucleic acid fragments. In applications where distinguishing between fine molecular weight differences is critical—such as PCR product analysis—standard agaroses often struggle to provide the necessary definition. This specialized formulation addresses that challenge, offering exceptional clarity and low background for fragments ranging from 20 bp to 800 bp.
Designed with sustainability and performance in mind, this agarose is manufactured using an innovative, organic solvent-free process, making it safer for the environment than traditional alternatives. Its intermediate melting point and low electroendoosmosis (EEO) ensure optimal gel structure and sample migration, while rigorous quality control guarantees a product free from DNase, RNase, and protease contamination. This makes it an indispensable tool for laboratories requiring reliable, high-definition results in molecular biology workflows.
Key Benefits:
- Ultra-High Resolution: Superior definition. Optimized for the separation and analysis of small nucleic acid fragments within the 20 bp to 800 bp range.
- High Clarity: Low background interference. Delivers crystal-clear gels with minimal background noise, ensuring accurate visualization of bands.
- Eco-Friendly Manufacturing: Green chemistry. Produced without the use of harmful organic solvents, reducing environmental impact and improving laboratory safety.
- Optimised Handling: Easy preparation. Features an intermediate melting point (≤70°C) and a gelling point (≤33°C) for convenient gel casting and manipulation.
- Purity Assured: Contaminant-free. Rigorously tested to be free from DNase, RNase, Protease, and Endonuclease activity, protecting valuable samples.
Technical Insights
Performance Data
In performance tests using a 2% HR Agarose gel (1x TAE) run at 5 V/cm for 2.5 hours, the matrix demonstrates exceptional separation capabilities. It effectively resolves a 20 bp DNA Ladder and a 50 bp High Ladder, distinguishing fragments clearly from 20 bp up to 1000 bp, with sharp banding particularly noted in the critical 20-800 bp range.
Specifications
- CAS Number: 39346-81-1
- Appearance: White to off-white powder
- Solubility: Clear colourless solution at 2g in 100ml water
- Gel Strength: ≥750 g/cm² (1.5% Gel)
- Gelling Point: ≤33°C (1.5% gel)
- Melting Point: ≤70°C (1.5% gel)
- EEO (Electroendoosmosis): ≤0.1%
- Sulfate: ≤0.1%
- Ash: ≤0.5%
- Nuclease & Protease Free: Yes (DNase, RNase, Protease, Endonuclease None Detected)