FastGene® Block & Go

FastGene® Block & Go: The 3-in-1 Blocker & Enhancer

FastGene® Block & Go serves as both a blocking solution and a signal enhancer for Western blot analysis. Its powerful, protein-free formulation reduces time-consuming and labor-intensive Western blot procedures.

This unique solution provides blocking, primary antibody, and secondary antibody hybridization in a single step. Furthermore, it enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates, leading to clearer, stronger results with minimal background.

Key Benefits:

• 3-in-1 Step Procedure: Combines blocking of the membrane, primary antibody, and secondary antibody in one step.
• Time-Saving: Saves up to 2.5 hours of hands-on-time compared to conventional protocols using milk or BSA.
• Enhanced Antibody Signal: Achieves a 2-5 fold increase in signal intensity for most protein targets.
• Increased Detection Sensitivity: Allows for the detection of proteins from as little as 0.5 µg of lysate.
• Protein-Free: Reduces overall background and minimizes non-specific signals, ideal for phospho-antibody detection.
• Flexible Use: Can be used in either single-step or two-step protocols.
• Less Hands-On Steps: Eliminates the need for 3 separate wash steps, reducing membrane handling.
• Broad Compatibility: Effective with any ECL substrate (HRP or AP) and compatible with both PVDF & NC membranes.

Supporting Data

Specifications

• Product: FastGene® Block & Go
• Pack Size: 500 mL
• Primary Use: Western blot blocking solution and signal enhancer
• Formulation: Protein-free
• Storage Conditions: Stable for up to 1 year at 25°C