Complete Denaturing Gradient Gel Electrophoresis System 20 x 20cm

Fully equipped WAVE system with temperature control unit, stirrer, gradient mixer and casting accessories to perform specific mutation analysis applications
Catalogue NumberDescriptionPack SizePrice
G9-1530Complete Denaturing Gradient Gel Electrophoresis System 20 x 20cmEach£4,341.00
Denaturing Gradient Gel Electrophoresis (DGGE) is an important technique used in the search for mutations and DNA polymorphisms critical in genetic disorders and cancers, and to understand genetic diversity among species. The new omniPAGE DGGE is a flexible system that can be fully customised to perform several different mutation detection techniques. it is a versatile single-unit solution for different single-base pair mutation detection methods. Using the innovative vertical screw-clamp technology of the new WAVE system, the system is fully equipped with temperature control unit, stirrer, and gradient mixer and casting accessories to perform specific mutation analysis applications. A powerful microprocessor-controlled PID temperature control unit enables various mutation detection techniques to be undertaken between ambient temperature and 70C, while the simple four screw design of the WAVE insert accelerates set up of denaturing PAGE gels. Accordingly it can be used to screen single-base pair changes in the following applications: Heteroduplex analysis (HA) Parallel Denaturing Gradient Gel Electrophoresis (DGGE) Constant Denaturing Gradient Gel Electrophoresis (CDGE) The flexibility provided by the modular design of the DGGE and its wide range of accessories enables laboratories to switch quickly and easily between different mutation detection techniques, thereby maximising throughput and screening efficiency. A maximum 96-sample throughput allows detection of as many mutations within a couple hours, alleviating many of the bottlenecks associated with screening for DNA mutations. GM100 Gradient Mixer ensures efficient gradient formation The GM100 gradient mixer is supplied as standard to ensure efficient gradient formation by mixing and delivering high- and low-density denaturant solutions. A flat-base design and support handle allows the GM100 to be secured to a retort stand, enabling it to be easily mounted on a magnetic stirring plate (e.g. CSL-STIR), while the mixing chamber can accommodate a magnetic stirrer to form a linear gradient. The system utilises novel vertical screw clamp technology to assemble two vertical gels. This reduces the number of screws required for set up making casting assembly faster, while a built-in inner buffer chamber within the PAGE insert allows set-up to be completed without the inclusion of heavy top tanks or buffer chambers. A dual purpose PAGE insert eliminates the need for plate transfer, and is used with a cam casting base to guarantee efficient leak free casting. The system can use all of the combs, glass plates and accessories of existing VS20 units, providing full flexibility. Two 1mm 24-sample combs are supplied as standard while optional 48-sample combs allow screening to take place directly from 96-Well thermal cycler blocks after PCR amplification. Complete with temperature control unit, cam casting base, glass plates with 1mm bonded spacers, 2x24-sample combs and gradient mixer -240 VAC version


G9-1530 Specification VS20WAVE-DGGE


G9-1530 Literature VS20WAVE-DGGE
G9-1530 Protocol VS20WAVE-DGGE
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