AccuGel 29:1: Precision & Safety for Electrophoresis
AccuGel 29:1 is a ready-to-use, 30% aqueous solution of acrylamide and bis-acrylamide, specifically formulated to eliminate the hazards and inconsistencies associated with weighing toxic powdered reagents. Traditional acrylamide powder poses a significant neurotoxic risk through inhalation of dust, and batch-to-batch variations in weighing can lead to irreproducible gel pore sizes. AccuGel solves these issues by providing a pre-mixed, ultra-pure liquid solution that ensures maximum safety for laboratory personnel while delivering reliable, high-resolution results every time.
At the heart of AccuGel’s superior performance is its unique gas-stabilised chemistry. Unlike standard solutions that rely on chemical inhibitors (which can interfere with polymerisation) or hydrolysis-prone additives, AccuGel utilises a gas stabilisation technology that prevents spontaneous polymerisation during storage. This ensures the solution maintains a crystal-clear shelf life of up to 24 months. The 29:1 cross-linker ratio is a versatile standard, optimised for the separation of nucleic acids (DNA/RNA) and proteins in standard SDS-PAGE applications.
Key Benefits:
- Enhanced Safety: Zero inhalation risk. By replacing powdered acrylamide with a liquid solution, you completely eliminate the risk of airborne neurotoxic dust, significantly improving laboratory health and safety standards.
- Gas Stabilised: Long-term reliability. The proprietary gas stabilisation prevents spontaneous polymerisation without interfering with the setting of the gel, ensuring consistent gelling times even after months of storage.
- Reproducible Results: Consistent pore size. Manufactured under strict quality controls from ultra-pure reagents, AccuGel ensures that every gel has the exact 29:1 matrix structure required for sharp, reproducible bands.
- Versatile Application: DNA & Protein separation. The 29:1 ratio (3.3% Crosslinker) is the industry standard for a wide range of molecular weights, suitable for both nucleic acid sequencing gels and SDS-PAGE protein analysis.
Technical Insights
AccuGel limits the accumulation of acrylic acid, a common breakdown product in acrylamide solutions that can cause electroendosmosis and band distortion. By minimising hydrolysis and eliminating metal ion contamination (which can alter polymerisation rates), AccuGel provides a neutral, stable matrix. The solution is 0.2 micron filtered to remove any particulate matter, ensuring a flawless gel structure critical for sensitive sequencing or blotting applications.
Specifications
- Composition: 30% (w/v) Acrylamide / Bis-Acrylamide
- Ratio: 29:1 (Acrylamide:Bis)
- Stabilisation: Gas-Stabilised (Reduced acrylic acid formation)
- Filtration: 0.2 µm filtered
- Storage: 4°C (Do not freeze)
- Shelf Life: 24 months (in original unopened bottle)
- Appearance: Clear, colourless liquid