This kit provides a rapid spin column procedure for the purification and concentration of up to 10 µg of oligonucleotides of various sizes. The kit is used for the purification of synthesized oligos, as well as the clean-up of oligos from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labelling.
Single or double stranded DNA or RNA oligos larger than 10 bp can be purified with this kit (not recommended for the removal of PCR primers). Oligos of up to 1000bp have been tested with this kit.
This kit purifies oligos from other reaction components including Proteins, buffers and nucleotides without the use of phenol, chloroform, alcohol precipitation or urea PAGE extraction.
The kit provides a high quality product with up to 90% recovery. The purified oligos can be used in a number of downstream applications including end-point or quantitative reverse transcription PCR, Northern blotting, in situ hybridization, and RNAi studies.