Saliva DNA Isolation Kit (96-Well Format): High-Throughput Non-Invasive Extraction
The Saliva DNA Isolation Kit (96-Well Format) provides a rapid and reliable method for the parallel purification of high-quality genomic DNA from both fresh and preserved saliva samples. As saliva becomes an increasingly preferred alternative to blood for non-invasive sample collection, researchers require a robust extraction method that effectively removes PCR inhibitors and stabilisers found in preservation buffers. This 96-well system is specifically engineered to handle high-volume screening, delivering DNA of the highest integrity suitable for sensitive downstream applications in diagnostics, epidemiology, and large-scale population studies.
The technology utilises advanced 96-well plate chromatography based on proprietary silica-resin technology, allowing for the simultaneous processing of up to 96 samples using either a vacuum manifold or centrifugation. The protocol begins with an efficient lysis step involving Proteinase K to disrupt cells and denature proteins, followed by the selective binding of DNA to the silica matrix within the plate wells. Impurities, salts, and proteins are washed away through a series of rapid wash steps, and the purified genomic DNA is eluted in a flexible volume. This system ensures a seamless workflow from collection to analysis, particularly when paired with high-throughput liquid handling platforms.
Key Benefits:
- High-Throughput Efficiency: 96 samples processed simultaneously. The plate-based format is ideal for large-scale genetic screening and biobanking, significantly increasing laboratory productivity.
- Versatile Protocol: Vacuum or centrifugation compatible. Fully compatible with standard vacuum manifolds and 96-well plate centrifuges, providing flexibility to suit existing laboratory infrastructure.
- Superior DNA Purity: Optimised for sensitive assays. Effectively removes inhibitors common to saliva samples, ensuring success in demanding applications such as Next-Generation Sequencing (NGS), qPCR, and microarray analysis.
- Non-Invasive Convenience: High donor compliance. Enables painless and cost-effective sample collection without the need for trained phlebotomists, ideal for remote or large-scale study recruitment.
- Broad Sample Compatibility: Fresh or preserved inputs. Validated for use with fresh saliva as well as samples stored in preservation buffers, ensuring stable DNA recovery even from aged samples.
Technical Insights
The Saliva DNA Isolation Kit (96-Well Format) is designed to provide consistent yields across the entire plate, minimising well-to-well variability. The purified DNA typically exhibits an A260/A280 ratio between 1.7 and 2.1, reflecting the high efficiency of the protein and contaminant removal process. This high-integrity DNA is suitable for long-term storage and remains stable for use in various genetic tests, including Single Nucleotide Polymorphism (SNP) genotyping and viral pathogen detection from saliva-based diagnostics.
Specifications
- Catalogue No: P4-0404
- Format: 96-Well Plate
- Sample Type: Fresh or Preserved Saliva
- Input Volume: Up to 0.5 mL (Preserved) or 0.25 mL (Fresh)
- Average DNA Yield: 3 – 7 µg (Donor dependent)
- Purification Chemistry: Silica-resin based
- Downstream Applications: NGS, qPCR, Microarray, SNP Genotyping, Southern Blot
- Compatibility: Vacuum manifold or centrifugation protocols
- Storage: Room Temperature (15-25°C)