P4-0450
Total RNA Purification Kit (CE/IVD Marked) – Dx17200

For rapid purification of total RNA - including microRNA - without phenol for in vitro diagnostic use. CE-IVD marked in accordance with EU Directive 98/79/EC
P4-0450 Image
Catalogue NumberDescriptionPack SizePrice
P4-0450Total RNA Purification Kit (CE/IVD Marked) – Dx1720050 Preps£297.50
  • CE-IVD marked in accordance with EU Directive 98/79/EC
  • Ideal for use in in vitro diagnostic workflows
  • Extract high quality & purity total RNA including miRNA
  • No phenol step required
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Efficiently extract small RNA irrespective of GC content
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Convenient & fast spin column format
  • Isolate from a wide variety of specimens
  • Purified RNA is suitable for a variety of downstream applications

Norgen’s Total RNA Purification Kit Dx provides a rapid method for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use.

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

To minimise irregularities in diagnostic results, suitable controls for downstream applications should be used.

Norgen’s Total RNA Purification Kit Dx is intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques.

Norgen’s Total RNA Purification Kit Dx does not provide a diagnostic result.

It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

Kit Specifications
Binding Capacity Per Column
Up to 50 μg RNA
Maximum Loading Volume Per Spin Column
650 μL
Size of RNA Purified
All sizes, including < 200 nt
Time to Complete 10 Purifications
20 minutes
Maximum Amount of Starting Material
Tissues
10 mg (for most tissues)
Blood
100 µL
Plasma/Serum
200 µL
Bacteria
1 x 10cells
Yeast
1 x 108 cells
Fungi
50 mg
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature.  All solutions and plastics can be used until the expiration date specified on their labels

Specification

Documentation

Literature
P4-0450 Literature Dx17200
Protocol
P4-0450 Protocol Dx17200
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