FFPE RNA/DNA Purification PLUS Kit – 54300

Sequential isolation and purification of total RNA and genomic DNA from FFPE tissue samples
Catalogue NumberDescriptionPack SizePrice
P4-0095FFPE RNA/DNA Purification PLUS Kit – 5430050 Preps£649.00
FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of Total RNA (including microRNA) and Genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. The RNA and DNA are sequentially purified into separate fractions using this kit. Using formalin to fix tissues leads to crosslinking of the nucleic acids and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the nucleic acids over time. FFPE RNA/DNA Purification Plus Kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of nucleic acids. The kit is able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. The RNA is purified from other cellular components without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. The purified Genomic DNA is also of the highest quality, and can be used in PCR reactions, sequencing, Southern blotting and SNP analysis.


P4-0095 Specification 54300


P4-0095 Literature 54300
P4-0095 Protocol 54300
Catalogue NumberDescriptionPack SizePrice
P4-0140RNA/DNA/Protein Purification Plus kit – 4770050 Preps£925
P4-0072FFPE RNA Purification Kit – 2530050 Preps£501
P4-0233FFPE RNA Purification Kit, 96-Well Format – 254002 Plates£1180
P4-0062Leukocyte RNA Purification Kit – 2120050 Preps£396